Insulin-like 3 affects zebrafish spermatogenic cells directly and via Sertoli cells.

Autor: Crespo D; Reproductive Biology Group, Division Developmental Biology, Department of Biology, Science Faculty, Utrecht University, Utrecht, The Netherlands.; Research Group Reproduction and Developmental Biology, Institute of Marine Research, Bergen, Norway., Assis LHC; Reproductive Biology Group, Division Developmental Biology, Department of Biology, Science Faculty, Utrecht University, Utrecht, The Netherlands., Zhang YT; State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Fujian, PR China.; Institute of Oceanography, Minjiang University, Fuzhou, PR China., Safian D; Reproductive Biology Group, Division Developmental Biology, Department of Biology, Science Faculty, Utrecht University, Utrecht, The Netherlands.; Experimental Zoology Group and Aquaculture and Fisheries Group, Department of Animal Science, Wageningen University, Wageningen, The Netherlands., Furmanek T; Research Group Reproduction and Developmental Biology, Institute of Marine Research, Bergen, Norway., Skaftnesmo KO; Research Group Reproduction and Developmental Biology, Institute of Marine Research, Bergen, Norway., Norberg B; Institute of Marine Research, Austevoll Research Station, Storebø, Norway., Ge W; Center of Reproduction, Development and Aging (CRDA), Faculty of Health Sciences, University of Macau, Taipa, Macau, China., Choi YC; Center of Reproduction, Development and Aging (CRDA), Faculty of Health Sciences, University of Macau, Taipa, Macau, China., den Broeder MJ; Division of Toxicology, Institute for Risk Assessment Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands., Legler J; Division of Toxicology, Institute for Risk Assessment Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands., Bogerd J; Reproductive Biology Group, Division Developmental Biology, Department of Biology, Science Faculty, Utrecht University, Utrecht, The Netherlands., Schulz RW; Reproductive Biology Group, Division Developmental Biology, Department of Biology, Science Faculty, Utrecht University, Utrecht, The Netherlands. r.w.schulz@uu.nl.; Research Group Reproduction and Developmental Biology, Institute of Marine Research, Bergen, Norway. r.w.schulz@uu.nl.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2021 Feb 15; Vol. 4 (1), pp. 204. Date of Electronic Publication: 2021 Feb 15.
DOI: 10.1038/s42003-021-01708-y
Abstrakt: Pituitary hormones can use local signaling molecules to regulate target tissue functions. In adult zebrafish testes, follicle-stimulating hormone (Fsh) strongly increases the production of insulin-like 3 (Insl3), a Leydig cell-derived growth factor found in all vertebrates. Little information is available regarding Insl3 function in adult spermatogenesis. The Insl3 receptors Rxfp2a and 2b were expressed by type A spermatogonia and Sertoli and myoid cells, respectively, in zebrafish testis tissue. Loss of insl3 increased germ cell apoptosis in males starting at 9 months of age, but spermatogenesis appeared normal in fully fertile, younger adults. Insl3 changed the expression of 409 testicular genes. Among others, retinoic acid (RA) signaling was up- and peroxisome proliferator-activated receptor gamma (Pparg) signaling was down-regulated. Follow-up studies showed that RA and Pparg signaling mediated Insl3 effects, resulting in the increased production of differentiating spermatogonia. This suggests that Insl3 recruits two locally active nuclear receptor pathways to implement pituitary (Fsh) stimulation of spermatogenesis.
Databáze: MEDLINE
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