Identification of additional outer segment targeting signals in zebrafish rod opsin.
| Autor: | Fang X; Bateson Centre and Department of Biomedical Science, University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK., Peden AA; Department of Biomedical Science, University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK., van Eeden FJM; Bateson Centre and Department of Biomedical Science, University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK f.j.vaneeden@sheffield.ac.uk., Malicki JJ; Bateson Centre and Department of Biomedical Science, University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of cell science [J Cell Sci] 2021 Mar 26; Vol. 134 (6). Date of Electronic Publication: 2021 Mar 26. |
| DOI: | 10.1242/jcs.254995 |
| Abstrakt: | In vertebrate photoreceptors, opsins are highly concentrated in a morphologically distinct ciliary compartment known as the outer segment (OS). Opsin is synthesized in the cell body and transported to the OS at a remarkable rate of 100 to 1000 molecules per second. Opsin transport defects contribute to photoreceptor loss and blindness in human ciliopathies. Previous studies revealed that the rhodopsin C-terminal tail, of 44 amino acids, is sufficient to mediate OS targeting in Xenopus photoreceptors. Here, we show that, although the Xenopus C-terminus retains this function in zebrafish, the homologous zebrafish sequence is not sufficient to target opsin to the OS. This functional difference is largely caused by a change of a single amino acid present in Xenopus but not in other vertebrates examined. Furthermore, we find that sequences in the third intracellular cytoplasmic loop (IC3) and adjacent regions of transmembrane helices 6 and 7 are also necessary for opsin transport in zebrafish. Combined with the cytoplasmic tail, these sequences are sufficient to target opsin to the ciliary compartment. Competing Interests: Competing interestsThe authors declare no competing or financial interests. (© 2021. Published by The Company of Biologists Ltd.) |
| Databáze: | MEDLINE |
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