Optoacoustic/Fluorescent/Acoustic Imaging Probe Based on Air-Filled Bubbles Functionalized with Gold Nanorods and Fluorescein Isothiocyanate.

Autor:	Barmin RA; Skolkovo Institute of Science and Technology, 3 Nobelya Str., Moscow 121205, Russia., Rudakovskaya PG; Skolkovo Institute of Science and Technology, 3 Nobelya Str., Moscow 121205, Russia., Chernyshev VS; Skolkovo Institute of Science and Technology, 3 Nobelya Str., Moscow 121205, Russia., Guslyakova OI; Saratov State University, 83 Astrakhanskaya Str., Saratov 410012, Russia., Belcov PA; Anta-Med Premium, LLC, 11 Derbenevskaya Naberezhnaya, Moscow 115114, Russia., Obukhova EN; Skolkovo Institute of Science and Technology, 3 Nobelya Str., Moscow 121205, Russia., Gayer AV; Lomonosov Moscow State University, 1/2 Leninskie Gory, Moscow 119991, Russia., Shirshin EA; Lomonosov Moscow State University, 1/2 Leninskie Gory, Moscow 119991, Russia.; Institute of Spectroscopy of the Russian Academy of Sciences, 5 Fizicheskaya Str., Troitsk, Moscow 108840, Russia.; Institute for Regenerative Medicine, Sechenov First Moscow State Medical University, Trubetskaya 8-2, Moscow 119048, Russia., Gorin DA; Skolkovo Institute of Science and Technology, 3 Nobelya Str., Moscow 121205, Russia.
Jazyk:	angličtina
Zdroj:	ACS omega [ACS Omega] 2021 Jan 25; Vol. 6 (5), pp. 3809-3821. Date of Electronic Publication: 2021 Jan 25 (Print Publication: 2021).
DOI:	10.1021/acsomega.0c05518
Abstrakt:	Liquid/surfactant/gas interfaces are promising objects for nanoengineered multimodal contrasts, which can be used for biomedical imaging in preclinical and clinical applications. Microbubbles with the gaseous core and shell made of lipids/proteins have already acted as ultrasound (US) contrast agents for angiography. In the present work, microbubbles with a shell composed of Span 60 and Tween 80 surfactants functionalized with fluorescein isothiocyanate and gold nanorods to achieve a multimodal combination of US, fluorescence, and optoacoustic imaging are described. Optimal conditions for microbubble generation by studying the surface tension of the initial solutions and analyzing the size, stability, and charge of the resulting bubbles were found. By controlling and modifying bubbles' surface properties, an increase in stability and storage time can be achieved. The functionalization of bubbles with gold nanoparticles and a dye by using an optimally selected sonication protocol was performed. The biomedical application's potential in imaging modalities of functionalized microbubbles using a medical US device with a frequency of 50 MHz, fluorescence tomography, and raster-scanning optoacoustic mesoscopy measurements was evaluated. The obtained results are important for optimum stabilization and functionalization of gas/liquid interfaces and the following applications in the multimodal biomedical imaging. Competing Interests: The authors declare no competing financial interest. (© 2021 The Authors. Published by American Chemical Society.)
Databáze:	MEDLINE
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