Cardioprotective effects of erythropoietin in diabetic rats determined by CD34 and vascular endothelial growth factor levels.

Autor:	Oztas ÝM; Department of Cardiovascular Surgery, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey.; Cardiovascular Surgery Clinic, Bagcilar Education and Research Hospital, Istanbul, Turkey., Meric M; Department of Cardiovascular Surgery, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey., Beyaz MO; Department of Cardiovascular Surgery, Medical Faculty, Medipol University, Istanbul, Turkey., Onalan MA; Department of Cardiovascular Surgery, Acibadem Mehmet Ali Aydinlar University, Atakent Hospital, Istanbul, Turkey., Sonmez K; Veterinary Faculty, University-Cerrahpasa, Istanbul, Turkey., Oter K; Veterinary Faculty, University-Cerrahpasa, Istanbul, Turkey., Ziyade S; Department of Thoracic Surgery, Bezmialem University, Istanbul, Turkey., Omer B; Department of Biochemistry, Medical Faculty, Istanbul University, Istanbul Turkey., Alpagut U; Department of Cardiovascular Surgery, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey., Ugurlucan M; Department of Cardiovascular Surgery, Medical Faculty, Medipol University, Istanbul, Turkey.
Jazyk:	angličtina
Zdroj:	Archives of medical sciences. Atherosclerotic diseases [Arch Med Sci Atheroscler Dis] 2020 Jan 19; Vol. 5, pp. e1-e12. Date of Electronic Publication: 2020 Jan 19 (Print Publication: 2020).
DOI:	10.5114/amsad.2020.92346
Abstrakt:	Introduction: In this study, the effects of diabetes mellitus on the cardiovascular system were investigated by assessing the stem cell levels in serum and heart and compared with the normal population. Additionally, efficacy of erythropoietin, which is known to increase stem cells, was studied in diabetic rats. Material and Methods: Twenty-five male Sprague Dawley rats were divided into three groups as a control group (group 1), diabetic group (group 2) and erythropoietin induced diabetic group (group 3). A diabetes model was created with streptozocin. In group 3 rats received 3000 U/kg of erythropoietin. At the end of 1 month blood reticulocyte levels, degree of tissue fibrosis and immunohistochemical assessment of reliable stem cell markers, CD34 and vascular endothelial growth factor (VEGF), were analyzed. Results: The increase in the blood glucose levels resulted in a significant decrease in reticulocyte levels in group 2. The increase in blood glucose levels resulted in a statistically significant increase in tissue level of fibrosis, CD34 and VEGF. When the rats in groups 1 and 2 were compared, the fibrosis, CD34 and VEGF levels were found to increase significantly. When group 2 and group 3 were compared, the amount of fibrosis was lower and the levels of CD34 and VEGF were significantly higher in group 3 than group 2. Conclusions: The results of our study indicated that the amount of CD34 and VEGF which function in cellular protection and tissue regeneration may be enhanced with safely applicable erythropoietin leading to increase in reticulocyte levels in serum, and CD34 and VEGF levels in right atrium, right ventricle, left atrium, and left ventricle as a protective mechanism in diabetic rats. Competing Interests: The authors declare no conflict of interest. (Copyright: © 2020 Termedia & Banach.)
Databáze:	MEDLINE
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