Multidrug Resistant Klebsiella pneumoniae ST101 Clone Survival Chain From Inpatients to Hospital Effluent After Chlorine Treatment.
| Autor: | Popa LI; Department of Botany and Microbiology, Faculty of Biology, University of Bucharest, Bucharest, Romania.; Research Institute of the University of Bucharest, Bucharest, Romania.; Department of Bioinformatics, National Institute of Research and Development for Biological Sciences, Bucharest, Romania., Gheorghe I; Department of Botany and Microbiology, Faculty of Biology, University of Bucharest, Bucharest, Romania.; Research Institute of the University of Bucharest, Bucharest, Romania., Barbu IC; Department of Botany and Microbiology, Faculty of Biology, University of Bucharest, Bucharest, Romania.; Research Institute of the University of Bucharest, Bucharest, Romania., Surleac M; Research Institute of the University of Bucharest, Bucharest, Romania.; National Institute for Infectious Diseases 'Matei Bals', Bucharest, Romania., Paraschiv S; National Institute for Infectious Diseases 'Matei Bals', Bucharest, Romania., Măruţescu L; Department of Botany and Microbiology, Faculty of Biology, University of Bucharest, Bucharest, Romania.; Research Institute of the University of Bucharest, Bucharest, Romania., Popa M; Research Institute of the University of Bucharest, Bucharest, Romania., Pîrcălăbioru GG; Research Institute of the University of Bucharest, Bucharest, Romania., Talapan D; National Institute for Infectious Diseases 'Matei Bals', Bucharest, Romania., Niţă M; National Research and Development Institute for Industrial Ecology (ECOIND), Bucharest, Romania., Streinu-Cercel A; National Institute for Infectious Diseases 'Matei Bals', Bucharest, Romania.; Department II - Infectious Diseases, University of Medicine and Pharmacy 'Carol Davila', Bucharest, Romania., Streinu-Cercel A; National Institute for Infectious Diseases 'Matei Bals', Bucharest, Romania.; Department II - Infectious Diseases, University of Medicine and Pharmacy 'Carol Davila', Bucharest, Romania., Oţelea D; National Institute for Infectious Diseases 'Matei Bals', Bucharest, Romania., Chifiriuc MC; Department of Botany and Microbiology, Faculty of Biology, University of Bucharest, Bucharest, Romania.; Research Institute of the University of Bucharest, Bucharest, Romania. |
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| Jazyk: | angličtina |
| Zdroj: | Frontiers in microbiology [Front Microbiol] 2021 Jan 11; Vol. 11, pp. 610296. Date of Electronic Publication: 2021 Jan 11 (Print Publication: 2020). |
| DOI: | 10.3389/fmicb.2020.610296 |
| Abstrakt: | In this paper we describe the transmission of a multi-drug resistant Klebsiella pneumoniae ST101 clone from hospital to wastewater and its persistence after chlorine treatment. Water samples from influents and effluents of the sewage tank of an infectious diseases hospital and clinical strains collected from the intra-hospital infections, during a period of 10 days prior to wastewater sampling were analyzed. Antibiotic resistant K. pneumoniae strains from wastewaters were recovered on selective media. Based on antibiotic susceptibility profiles and PCR analyses of antibiotic resistance (AR) genetic background, as well as whole-genome sequencing (Illumina MiSeq) and subsequent bioinformatic analyses, 11 ST101 K. pneumoniae strains isolated from hospital wastewater influent, wastewater effluent and clinical sector were identified as clonally related. The SNP and core genome analyses pointed out that five strains were found to be closely related (with ≤18 SNPs and identical cgMLST profile). The strains belonging to this clone harbored multiple acquired AR genes [ bla Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Popa, Gheorghe, Barbu, Surleac, Paraschiv, Măruţescu, Popa, Pîrcălăbioru, Talapan, Niţă, Streinu-Cercel, Streinu-Cercel, Oţelea and Chifiriuc.) |
| Databáze: | MEDLINE |
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