Genomics accurately predicts antimicrobial resistance in Staphylococcus pseudintermedius collected as part of Vet-LIRN resistance monitoring.

Autor: Tyson GH; U.S. Food and Drug Administration, Center for Veterinary Medicine, Office of Research, United States. Electronic address: Gregory.Tyson@fda.hhs.gov., Ceric O; U.S. Food and Drug Administration, Center for Veterinary Medicine, Office of Research, United States., Guag J; U.S. Food and Drug Administration, Center for Veterinary Medicine, Office of Research, United States., Nemser S; U.S. Food and Drug Administration, Center for Veterinary Medicine, Office of Research, United States., Borenstein S; U.S. Food and Drug Administration, Center for Veterinary Medicine, Office of Research, United States., Slavic D; University of Guelph - Animal Health Laboratory, Canada., Lippert S; University of Guelph - Animal Health Laboratory, Canada., McDowell R; University of Guelph - Animal Health Laboratory, Canada., Krishnamurthy A; University of Guelph - Animal Health Laboratory, Canada., Korosec S; Manitoba Agriculture and Resource Development - Veterinary Diagnostic Services, Canada., Friday C; Manitoba Agriculture and Resource Development - Veterinary Diagnostic Services, Canada., Pople N; Manitoba Agriculture and Resource Development - Veterinary Diagnostic Services, Canada., Saab ME; Diagnostic Services, Atlantic Veterinary College, University of Prince Edward Island, Canada., Fairbrother JH; Complexe de diagnostic et d'épidémiosurveillance vétérinaires du Québec, Canada., Janelle I; Complexe de diagnostic et d'épidémiosurveillance vétérinaires du Québec, Canada., McMillan D; University of Saskatchewan - Prairie Diagnostic Services Inc, Canada., Bommineni YR; Bronson Animal Disease Diagnostic Laboratory, United States., Simon D; Bronson Animal Disease Diagnostic Laboratory, United States., Mohan S; Bronson Animal Disease Diagnostic Laboratory, United States., Sanchez S; Athens Veterinary Diagnostic Laboratory, College of Veterinary Medicine, The University of Georgia, United States., Phillips A; Athens Veterinary Diagnostic Laboratory, College of Veterinary Medicine, The University of Georgia, United States., Bartlett P; Athens Veterinary Diagnostic Laboratory, College of Veterinary Medicine, The University of Georgia, United States., Naikare H; University of Georgia - Tifton Veterinary Diagnostic & Investigational Laboratory, United States., Watson C; University of Georgia - Tifton Veterinary Diagnostic & Investigational Laboratory, United States., Sahin O; Iowa State University, United States., Stinman C; Iowa State University, United States., Wang L; University of Illinois Veterinary Diagnostic Laboratory - College of Veterinary Medicine, United States., Maddox C; University of Illinois Veterinary Diagnostic Laboratory - College of Veterinary Medicine, United States., DeShambo V; University of Illinois Veterinary Diagnostic Laboratory - College of Veterinary Medicine, United States., Hendrix K; Indiana Animal Disease Diagnostic Laboratory, United States., Lubelski D; Indiana Animal Disease Diagnostic Laboratory, United States., Burklund A; Kansas State University, United States., Lubbers B; Kansas State University, United States., Reed D; Murray State University Breathitt Veterinary Center, United States., Jenkins T; Murray State University Breathitt Veterinary Center, United States., Erol E; University of Kentucky, United States., Patel M; University of Kentucky, United States., Locke S; University of Kentucky, United States., Fortner J; University of Kentucky, United States., Peak L; Louisiana State University, United States., Balasuriya U; Louisiana State University, United States., Mani R; Michigan State University, United States., Kettler N; Michigan State University, United States., Olsen K; University of Minnesota Veterinary Diagnostic Lab, United States., Zhang S; University of Missouri Veterinary Medical Diagnostic Laboratory, United States., Shen Z; University of Missouri Veterinary Medical Diagnostic Laboratory, United States., Landinez MP; Mississippi State University, Veterinary Research and Diagnostic Lab System, United States., Thornton JK; Mississippi State University, Veterinary Research and Diagnostic Lab System, United States., Thachil A; North Carolina Veterinary Diagnostic Lab System, United States., Byrd M; North Carolina State University, United States., Jacob M; North Carolina State University, United States., Krogh D; North Dakota State University Veterinary Diagnostic Laboratory, United States., Webb B; North Dakota State University Veterinary Diagnostic Laboratory, United States., Schaan L; North Dakota State University Veterinary Diagnostic Laboratory, United States., Patil A; New Jersey Department of Agriculture, Animal Health Diagnostic Laboratory, United States., Dasgupta S; New Jersey Department of Agriculture, Animal Health Diagnostic Laboratory, United States., Mann S; New Jersey Department of Agriculture, Animal Health Diagnostic Laboratory, United States., Goodman LB; Cornell University, College of Veterinary Medicine, United States., Franklin-Guild RJ; Cornell University, College of Veterinary Medicine, United States., Anderson RR; Cornell University, College of Veterinary Medicine, United States., Mitchell PK; Cornell University, College of Veterinary Medicine, United States., Cronk BD; Cornell University, College of Veterinary Medicine, United States., Aprea M; Cornell University, College of Veterinary Medicine, United States., Cui J; Ohio Animal Disease Diagnostic Lab, United States., Jurkovic D; Ohio Animal Disease Diagnostic Lab, United States., Prarat M; Ohio Animal Disease Diagnostic Lab, United States., Zhang Y; Ohio Animal Disease Diagnostic Lab, United States., Shiplett K; Ohio Animal Disease Diagnostic Lab, United States., Campos DD; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, United States., Rubio JVB; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, United States., Ramanchandran A; Oklahoma Animal Disease Diagnostic Laboraotry, College of Veterinary Medicine, Oklahoma State University, United States., Talent S; Oklahoma Animal Disease Diagnostic Laboraotry, College of Veterinary Medicine, Oklahoma State University, United States., Tewari D; PA Veterinary Laboratory, Pennsylvania Department of Agriculture, United States., Thirumalapura N; PA Veterinary Laboratory, Pennsylvania Department of Agriculture, United States., Kelly D; University of Pennsylvania, New Bolton Center, United States., Barnhart D; University of Pennsylvania, New Bolton Center, United States., Hall L; University of Pennsylvania, New Bolton Center, United States., Rankin S; University of Pennsylvania, Ryan Veterinary Hospital, United States., Dietrich J; University of Pennsylvania, Ryan Veterinary Hospital, United States., Cole S; University of Pennsylvania, Ryan Veterinary Hospital, United States., Scaria J; Animal Disease Research and Diagnostic Laboratory, South Dakota State University, United States., Antony L; Animal Disease Research and Diagnostic Laboratory, South Dakota State University, United States., Lawhon SD; Texas A&M University, College of Veterinary Medicine & Biomedical Sciences, Department of Veterinary Pathobiology, United States., Wu J; Texas A&M University, College of Veterinary Medicine & Biomedical Sciences, Department of Veterinary Pathobiology, United States., McCoy C; Virginia Department of Agriculture and Consumer Services- Lynchburg Regional Animal Health Laboratory, United States., Dietz K; Virginia Department of Agriculture and Consumer Services- Lynchburg Regional Animal Health Laboratory, United States., Wolking R; Washington State University, United States., Alexander T; Washington State University, United States., Burbick C; Washington State University, United States., Reimschuessel R; U.S. Food and Drug Administration, Center for Veterinary Medicine, Office of Research, United States.
Jazyk: angličtina
Zdroj: Veterinary microbiology [Vet Microbiol] 2021 Mar; Vol. 254, pp. 109006. Date of Electronic Publication: 2021 Feb 04.
DOI: 10.1016/j.vetmic.2021.109006
Abstrakt: Whole-genome sequencing (WGS) has changed our understanding of bacterial pathogens, aiding outbreak investigations and advancing our knowledge of their genetic features. However, there has been limited use of genomics to understand antimicrobial resistance of veterinary pathogens, which would help identify emerging resistance mechanisms and track their spread. The objectives of this study were to evaluate the correlation between resistance genotypes and phenotypes for Staphylococcus pseudintermedius, a major pathogen of companion animals, by comparing broth microdilution antimicrobial susceptibility testing and WGS. From 2017-2019, we conducted antimicrobial susceptibility testing and WGS on S. pseudintermedius isolates collected from dogs in the United States as a part of the Veterinary Laboratory Investigation and Response Network (Vet-LIRN) antimicrobial resistance monitoring program. Across thirteen antimicrobials in nine classes, resistance genotypes correlated with clinical resistance phenotypes 98.4 % of the time among a collection of 592 isolates. Our findings represent isolates from diverse lineages based on phylogenetic analyses, and these strong correlations are comparable to those from studies of several human pathogens such as Staphylococcus aureus and Salmonella enterica. We uncovered some important findings, including that 32.3 % of isolates had the mecA gene, which correlated with oxacillin resistance 97.0 % of the time. We also identified a novel rpoB mutation likely encoding rifampin resistance. These results show the value in using WGS to assess antimicrobial resistance in veterinary pathogens and to reveal putative new mechanisms of resistance.
(Published by Elsevier B.V.)
Databáze: MEDLINE
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