Intra-articular treatment with hyaluronic acid associated with gold nanoparticles in a mechanical osteoarthritis model in Wistar rats.

Autor: Filho MCB; Laboratory of Experimental Phisiopatology, Program of postgraduate in Science of Health, Universidade do Extremo Sul Catarinense, Criciúma, Santa Catarina, Brazil., Dos Santos Haupenthal DP; Laboratory of Experimental Phisiopatology, Program of postgraduate in Science of Health, Universidade do Extremo Sul Catarinense, Criciúma, Santa Catarina, Brazil., Zaccaron RP; Laboratory of Experimental Phisiopatology, Program of postgraduate in Science of Health, Universidade do Extremo Sul Catarinense, Criciúma, Santa Catarina, Brazil., de Bem Silveira G; Laboratory of Experimental Phisiopatology, Program of postgraduate in Science of Health, Universidade do Extremo Sul Catarinense, Criciúma, Santa Catarina, Brazil., de Roch Casagrande L; Laboratory of Experimental Phisiopatology, Program of postgraduate in Science of Health, Universidade do Extremo Sul Catarinense, Criciúma, Santa Catarina, Brazil., Lupselo FS; Laboratory of Experimental Phisiopatology, Program of postgraduate in Science of Health, Universidade do Extremo Sul Catarinense, Criciúma, Santa Catarina, Brazil., Alves N; Graduate Program of Biomedical Sciences, University Center of Herminio Ometto Foundation, Araras, São Paulo, Brazil., de Sousa Mariano S; Graduate Program of Biomedical Sciences, University Center of Herminio Ometto Foundation, Araras, São Paulo, Brazil., do Bomfim FRC; University Center of Herminio Ometto Foundation, Araras, São Paulo, Brazil., de Andrade TAM; Graduate Program of Biomedical Sciences, University Center of Herminio Ometto Foundation, Araras, São Paulo, Brazil., Machado-de-Ávila RA; Laboratory of Experimental Phisiopatology, Program of postgraduate in Science of Health, Universidade do Extremo Sul Catarinense, Criciúma, Santa Catarina, Brazil., Silveira PCL; Laboratory of Experimental Phisiopatology, Program of postgraduate in Science of Health, Universidade do Extremo Sul Catarinense, Criciúma, Santa Catarina, Brazil.
Jazyk: angličtina
Zdroj: Journal of orthopaedic research : official publication of the Orthopaedic Research Society [J Orthop Res] 2021 Dec; Vol. 39 (12), pp. 2546-2555. Date of Electronic Publication: 2021 Apr 29.
DOI: 10.1002/jor.25008
Abstrakt: This study aimed to evaluate the effects of intra-articular treatment with hyaluronic acid (HA) associated with GNPs in a mechanical model of osteoarthritis induced by median meniscectomy (MM). Fifty Wistar rats (2 months weighing between 250 and 300 g) were used, divided into five groups of 10 animals each: Sham, osteoarthritis (OA), OA + HA, OA + gold nanoparticles (GNPs), and OA + HA + GNPs. Intra-articular treatment was started 30 days after the model was induced, with a frequency of 2 weeks for 60 days. Fifteen days after the last application, the animals were euthanized with the removal of the joint tissue for biochemical and histological analysis. The model used was able to mimic osteoarthritis, characterized by the presence of high levels of proinflammatory cytokines, oxidative stress, and degeneration of joint surfaces (Grade III, according to SCORE OARSI). The isolated use of HA or GNPs provided beneficial results to the joint; however, only the group subjected to the association between HA and GNPs showed the attenuation of oxidative stress and reduced proinflammatory markers, with a simultaneous increase in levels of anti-inflammatory cytokines and growth factors. Upon histological analysis, only the OA + HA + GNPs group achieved the restoration of the thickness of the joint cartilage with reduced damage and return to the intact joint surface. The results found demonstrated that the association of GNPs with HA was able to reverse the deleterious effects caused by the model by inhibiting the progressive degeneration of joint surfaces, representing a promising treatment for osteoarthritis.
(© 2021 Orthopaedic Research Society. Published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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