Three-dimensional vascular microenvironment landscape in human glioblastoma.

Autor: Cribaro GP; Department of Biochemistry and Molecular Biology, Institut de Neurociències, School of Medicine, Lab M2-107, Office M2-117, Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain., Saavedra-López E; Department of Biochemistry and Molecular Biology, Institut de Neurociències, School of Medicine, Lab M2-107, Office M2-117, Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain., Romarate L; Department of Biochemistry and Molecular Biology, Institut de Neurociències, School of Medicine, Lab M2-107, Office M2-117, Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain., Mitxitorena I; Department of Biochemistry and Molecular Biology, Institut de Neurociències, School of Medicine, Lab M2-107, Office M2-117, Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain., Díaz LR; Department of Biochemistry and Molecular Biology, Institut de Neurociències, School of Medicine, Lab M2-107, Office M2-117, Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain., Casanova PV; Department of Biochemistry and Molecular Biology, Institut de Neurociències, School of Medicine, Lab M2-107, Office M2-117, Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain., Roig-Martínez M; Department of Biochemistry and Molecular Biology, Institut de Neurociències, School of Medicine, Lab M2-107, Office M2-117, Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain., Gallego JM; Department of Neurosurgery & Department of Pathology, Valencia General Hospital, Valencia, Spain., Perez-Vallés A; Department of Neurosurgery & Department of Pathology, Valencia General Hospital, Valencia, Spain., Barcia C; Department of Biochemistry and Molecular Biology, Institut de Neurociències, School of Medicine, Lab M2-107, Office M2-117, Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain. carlos.barcia@uab.es.
Jazyk: angličtina
Zdroj: Acta neuropathologica communications [Acta Neuropathol Commun] 2021 Feb 12; Vol. 9 (1), pp. 24. Date of Electronic Publication: 2021 Feb 12.
DOI: 10.1186/s40478-020-01115-0
Abstrakt: The cellular complexity of glioblastoma microenvironments is still poorly understood. In-depth, cell-resolution tissue analyses of human material are rare but highly necessary to understand the biology of this deadly tumor. Here we present a unique 3D visualization revealing the cellular composition of human GBM in detail and considering its critical association with the neo-vascular niche. Our images show a complex vascular map of human 3D biopsies with increased vascular heterogeneity and altered spatial relationship with astrocytes or glioma-cell counterparts. High-resolution analysis of the structural layers of the blood brain barrier showed a multilayered fenestration of endothelium and basement membrane. Careful examination of T cell position and migration relative to vascular walls revealed increased infiltration corresponding with tumor proliferation. In addition, the analysis of the myeloid landscape not only showed a volumetric increase in glioma-associated microglia and macrophages relative to GBM proliferation but also revealed distinct phenotypes in tumor nest and stroma. Images and data sets are available on demand as a resource for public access.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje