Genotyping tools and resources to assess peanut germplasm: smut-resistant landraces as a case study.

Autor:	Massa AN; National Peanut Research Laboratory, USDA-ARS, Dawson, GA, USA., Bressano M; Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba, Córdoba, Argentina., Soave JH; Criadero El Carmen, General Cabrera, Córdoba, Argentina., Buteler MI; Criadero El Carmen, General Cabrera, Córdoba, Argentina., Seijo G; Instituto de Botánica del Nordeste (IBONE, CONICET-UNNE) and Facultad de Ciencias Exactas y Naturales y Agrimensura, Universidad Nacional del Nordeste, Corrientes, Argentina., Sobolev VS; National Peanut Research Laboratory, USDA-ARS, Dawson, GA, USA., Orner VA; National Peanut Research Laboratory, USDA-ARS, Dawson, GA, USA., Oddino C; Criadero El Carmen, General Cabrera, Córdoba, Argentina., Soave SJ; Criadero El Carmen, General Cabrera, Córdoba, Argentina., Faustinelli PC; National Peanut Research Laboratory, USDA-ARS, Dawson, GA, USA., de Blas FJ; Instituto Multidisciplinario de Biología Vegetal-(IMBIV-CONICET-UNC), Córdoba, Argentina., Lamb MC; National Peanut Research Laboratory, USDA-ARS, Dawson, GA, USA., Arias RS; National Peanut Research Laboratory, USDA-ARS, Dawson, GA, USA.
Jazyk:	angličtina
Zdroj:	PeerJ [PeerJ] 2021 Jan 29; Vol. 9, pp. e10581. Date of Electronic Publication: 2021 Jan 29 (Print Publication: 2021).
DOI:	10.7717/peerj.10581
Abstrakt:	Peanut smut caused by Thecaphora frezii is a severe fungal disease currently endemic to Argentina and Brazil. The identification of smut resistant germplasm is crucial in view of the potential risk of a global spread. In a recent study, we reported new sources of smut resistance and demonstrated its introgression into elite peanut cultivars. Here, we revisited one of these sources (line I0322) to verify its presence in the U.S. peanut germplasm collection and to identify single nucleotide polymorphisms (SNPs) potentially associated with resistance. Five accessions of Arachis hypogaea subsp. fastigiata from the U.S. peanut collection, along with the resistant source and derived inbred lines were genotyped with a 48K SNP peanut array. A recently developed SNP genotyping platform called RNase H2 enzyme-based amplification (rhAmp) was further applied to validate selected SNPs in a larger number of individuals per accession. More than 14,000 SNPs and nine rhAmp assays confirmed the presence of a germplasm in the U.S. peanut collection that is 98.6% identical ( P < 0.01, bootstrap t -test) to the resistant line I0322. We report this germplasm with accompanying genetic information, genotyping data, and diagnostic SNP markers. Competing Interests: The authors declare that they have no competing interests. Juan H. Soave, Mario I. Buteler, Claudio Oddino & Sara J. Soave are employed by Criadero El Carmen.
Databáze:	MEDLINE
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