Establishment and Application of a Dual TaqMan Real-Time PCR Method for Proteus Mirabilis and Proteus Vulgaris .

Autor:	Yang R; Chongqing Academy of Animal Science, Chongqing, China., Xu G; Chongqing Academy of Animal Science, Chongqing, China., Wang X; Chongqing Academy of Animal Science, Chongqing, China., Qing Z; Chongqing Nanchuan Animal Disease Prevention And Control Center, Chongqing, China., Fu L; Chongqing Academy of Animal Science, Chongqing, China.
Jazyk:	angličtina
Zdroj:	Polish journal of microbiology [Pol J Microbiol] 2020 Sep; Vol. 69 (3), pp. 293-300. Date of Electronic Publication: 2020 Aug 25.
DOI:	10.33073/pjm-2020-032
Abstrakt:	Proteus species are common opportunistic bacteria and foodborne pathogens. The proper detection of Proteus can effectively reduce the occurrence of food-borne public health events. Proteus mirabilis and Proteus vulgaris are the two most important pathogens in the Proteus genus. In this study, a dual TaqMan Real-Time PCR method was established to simultaneously detect and distinguish P. mirabilis and P. vulgaris in samples. The method exhibited good specificity, stability, and sensitivity. Specifically, the minimum detection concentrations of P. mirabilis and P. vulgaris in pure bacterial cultures were 6.08 × 10 2 colony forming units (CFU)/ml and 4.46 × 10 2 CFU/ml, respectively. Additionally, the minimum detectable number of P. mirabilis and P. vulgaris in meat and milk was 10 3 CFU/g. In addition, the method can be used to distinguish between strains of P. mirabilis and P. vulgaris within two hours. Overall, it is a sensitive, easy-to-use, and practical test for the identification and classification of Proteus in food. Competing Interests: Conflict of interest The authors do not report any financial or personal connections with other persons or organizations, which might negatively affect the contents of this publication and/or claim authorship rights to this publication. (© 2020 Rui Yang et al.)
Databáze:	MEDLINE
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