| Autor: |
Petrovskis I; Latvian Biomedical Research and Study Centre, Ratsupites 1, LV-1067 Riga, Latvia., Lieknina I; Latvian Biomedical Research and Study Centre, Ratsupites 1, LV-1067 Riga, Latvia., Dislers A; Latvian Biomedical Research and Study Centre, Ratsupites 1, LV-1067 Riga, Latvia., Jansons J; Latvian Biomedical Research and Study Centre, Ratsupites 1, LV-1067 Riga, Latvia., Bogans J; Latvian Biomedical Research and Study Centre, Ratsupites 1, LV-1067 Riga, Latvia., Akopjana I; Latvian Biomedical Research and Study Centre, Ratsupites 1, LV-1067 Riga, Latvia., Zakova J; Latvian Biomedical Research and Study Centre, Ratsupites 1, LV-1067 Riga, Latvia., Sominskaya I; Latvian Biomedical Research and Study Centre, Ratsupites 1, LV-1067 Riga, Latvia. |
| Abstrakt: |
The core proteins (HBc) of the hepatitis B virus (HBV) genotypes A, B, C, D, E, F, and G were cloned and expressed in Escherichia coli (E. coli ), and HBc-formed virus-like particles (VLPs) were purified with ammonium sulfate precipitation, gel filtration, and ion exchange chromatography (IEX). The best VLP yield was found for the HBc of the HBV genotypes D and G. For the HBc of the HBV genotypes D, F, and G, the possibility of dissociation and reassociation maintaining the native HBc structure was demonstrated. Single-stranded (ss) and double-stranded (ds) ribonucleic acid (RNA) was successfully packed into HBc VLPs for the HBV genotypes D and G. |
