| Autor: |
Maliński MP; Chair and Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, 14 Sw. Marii Magdaleny St., 61-861 Poznań, Poland., Budzianowski J; Chair and Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, 14 Sw. Marii Magdaleny St., 61-861 Poznań, Poland., Kikowska M; Chair and Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, 14 Sw. Marii Magdaleny St., 61-861 Poznań, Poland., Derda M; Chair and Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, 10 Fredry St., 61-701 Poznań, Poland., Jaworska MM; Chair and Department of Genetics and Pharmaceutical Microbiology, Poznan University of Medical Sciences, 4 Święcickiego St., 60-781 Poznań, Poland., Mlynarczyk DT; Chair and Department of Chemical Technology of Drugs, Poznan University of Medical Sciences, 6 Grunwaldzka St., 60-780 Poznań, Poland., Szukalska M; Laboratory of Environmental Research, Chair and Department of Toxicology, Poznan University of Medical Sciences, 30 Dojazd St., 60-631 Poznań, Poland., Florek E; Laboratory of Environmental Research, Chair and Department of Toxicology, Poznan University of Medical Sciences, 30 Dojazd St., 60-631 Poznań, Poland., Thiem B; Chair and Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, 14 Sw. Marii Magdaleny St., 61-861 Poznań, Poland. |
| Abstrakt: |
Genetically uniform plant material, derived from Lychnis flos-cuculi propagated in vitro, was used for the isolation of 20-hydroxyecdysone and polypodine B and subjected to an evaluation of the antifungal and antiamoebic activity. The activity of 80% aqueous methanolic extracts, their fractions, and isolated ecdysteroids were studied against pathogenic Acanthamoeba castellani . Additionally, a Microtox ® acute toxicity assay was performed. It was found that an 80% methanolic fraction of root extract exerts the most potent amoebicidal activity at IC 50 of 0.06 mg/mL at the 3rd day of treatment. Both ecdysteroids show comparable activity at IC 50 of 0.07 mg/mL. The acute toxicity of 80% fractions at similar concentrations is significantly higher than that of 40% fractions. Crude extracts exhibited moderate antifungal activity, with a minimum inhibitory concentration (MIC) within the range of 1.25-2.5 mg/mL. To the best of our knowledge, the present report is the first to show the biological activity of L. flos-cuculi in terms of the antifungal and antiamoebic activities and acute toxicity. It is also the first isolation of the main ecdysteroids from L. flos-cuculi micropropagated, ecdysteroid-rich plant material. |
