Transsynaptic mapping of Drosophila mushroom body output neurons.

Autor:	Scaplen KM; Department of Neuroscience, Brown University, Providence, United States.; Department of Psychology, Bryant University, Smithfield, United States.; Center for Health and Behavioral Sciences, Bryant University, Smithfield, United States., Talay M; Department of Neuroscience, Brown University, Providence, United States., Fisher JD; Department of Neuroscience, Brown University, Providence, United States., Cohn R; Laboratory of Neurophysiology and Behavior, The Rockefeller University, New York, United States., Sorkaç A; Department of Neuroscience, Brown University, Providence, United States., Aso Y; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Barnea G; Department of Neuroscience, Brown University, Providence, United States., Kaun KR; Department of Neuroscience, Brown University, Providence, United States.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2021 Feb 11; Vol. 10. Date of Electronic Publication: 2021 Feb 11.
DOI:	10.7554/eLife.63379
Abstrakt:	The mushroom body (MB) is a well-characterized associative memory structure within the Drosophila brain. Analyzing MB connectivity using multiple approaches is critical for understanding the functional implications of this structure. Using the genetic anterograde transsynaptic tracing tool, trans- Tango, we identified divergent projections across the brain and convergent downstream targets of the MB output neurons (MBONs). Our analysis revealed at least three separate targets that receive convergent input from MBONs: other MBONs, the fan-shaped body (FSB), and the lateral accessory lobe (LAL). We describe, both anatomically and functionally, a multilayer circuit in which inhibitory and excitatory MBONs converge on the same genetic subset of FSB and LAL neurons. This circuit architecture enables the brain to update and integrate information with previous experience before executing appropriate behavioral responses. Our use of trans -Tango provides a genetically accessible anatomical framework for investigating the functional relevance of components within these complex and interconnected circuits. Competing Interests: KS, MT, JF, RC, AS, YA, GB, KK No competing interests declared (© 2021, Scaplen et al.)
Databáze:	MEDLINE
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