Whey protein enriched with Stevia rebaudiana fraction restores the pancreatic function of streptozotocin induced diabetic rats.

Autor: Milani PG; Department of Biochemistry, State University of Maringá, Maringá, PR Brazil., Piovan S; Department of Biotechnology, Genetics and Cell Biology, State University of Maringá, Maringá, PR Brazil., Lima YC; Post-Graduate Program in Sciences (Human Physiology), State University of Sao Paulo, São Paulo, SP Brazil., Zorzenon MRT; Department of Biochemistry, State University of Maringá, Maringá, PR Brazil., da Rosa CVD; University North of Paraná, UNOPAR, Bandeirantes, PR Brazil., Peixoto GML; Department of Physiological Sciences, State University of Maringá, Maringá, PR Brazil., de Freitas Mathias PC; Department of Biotechnology, Genetics and Cell Biology, State University of Maringá, Maringá, PR Brazil., Natali MRM; Departament of Morphological Sciences, State University of Maringa, Maringá, PR Brazil., da Costa SC; Department of Biochemistry, State University of Maringá, Maringá, PR Brazil., Mareze-Costa CE; Department of Physiological Sciences, State University of Maringá, Maringá, PR Brazil.
Jazyk: angličtina
Zdroj: Journal of food science and technology [J Food Sci Technol] 2021 Feb; Vol. 58 (2), pp. 805-810. Date of Electronic Publication: 2020 Sep 25.
DOI: 10.1007/s13197-020-04799-3
Abstrakt: The study aims to analyse the treatment of whey protein enriched with Stevia rebaudiana fraction in insulin secretion and its role mitigating streptozotocin-induced hyperglycemia in rats. Thus, diabetic animals were treated with whey protein enriched with S. rebaudiana fraction or with only the protein isolate or only the Stevia fraction. Insulin level in plasma was measured by radioimmunoassay and the viability of β cells was detected by immunohistochemistry. The results showed that diabetic animals treated with whey protein enriched with S. rebaudiana fraction had a greater recovery from insulinemia, with plasma levels similar to non-diabetic animals (~ 0.13 ng/mL). In addition, the same group showed a higher number of insulin-positive pancreatic B cells (~ 66%) in immunohistochemistry analysis, while the diabetic groups treated with only the fraction of stevia or whey protein showed 38 and 59% of positive cells, respectively. These results show that the treatment may have restored the viability of streptozotocin-injured pancreatic B cells, and consequently increased insulin secretion, suggesting whey protein enriched with S. rebaudiana fraction can be used an adjunct/supplement in diabetic treatment.
Competing Interests: Conflicts of interestThere is no conflict of interest regarding the publication of this paper.
(© Association of Food Scientists & Technologists (India) 2020.)
Databáze: MEDLINE
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