Polycyclic Aromatic Hydrocarbon-DNA Adducts in Gulf of Mexico Sperm Whale Skin Biopsies Collected in 2012.

Autor:	Poirier MC; Carcinogen-DNA Interactions Section, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892-4255, USA., Marsili L; Department of Earth, Environmental and Physical Sciences, University of Siena, Via Mattioli 4, Siena 53100, Italy., Fossi MC; Department of Earth, Environmental and Physical Sciences, University of Siena, Via Mattioli 4, Siena 53100, Italy., Godard-Codding CAJ; Department of Environmental Toxicology, Institute of Environmental and Human Health, Texas Tech University, Lubbock, Texas 79409-1163, USA., Hernandez-Ramon EE; Carcinogen-DNA Interactions Section, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892-4255, USA., Si N; Carcinogen-DNA Interactions Section, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892-4255, USA., Divi KV; Carcinogen-DNA Interactions Section, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892-4255, USA., Divi RL; Methods and Technologies Branch, Division of Cancer Control and Population Sciences, National Cancer Institute, NIH, 6909 Medical Center Dr., Rockville, Maryland 20850, USA., Kerr I; Ocean Alliance, 32 Horton St., Gloucester, Maryland 01930, USA., Wise JP; Department of Pediatrics, University of Louisville School of Medicine, 571 S. Floyd St., Louisville, Kentucky 40292, USA.; Wise Laboratory of Environmental and Genetic Toxicology, Department of Pharmacology and Toxicology, University of Louisville School of Medicine, 505 S. Preston St., Louisville, Louisville, Kentucky 40292, USA., Wise CF; Department of Biological Sciences, North Carolina State University, 850 Main Campus Drive Raleigh, North Carolina 27606, USA.; Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27607, USA., Wise SS; Wise Laboratory of Environmental and Genetic Toxicology, Department of Pharmacology and Toxicology, University of Louisville School of Medicine, 505 S. Preston St., Louisville, Louisville, Kentucky 40292, USA., Aboueissa AE; Department of Mathematics and Statistics, University of Southern Maine, 96 Falmouth St., Portland, Maine 04104-9300, USA., Wise JTF; Wise Laboratory of Environmental and Genetic Toxicology, Department of Pharmacology and Toxicology, University of Louisville School of Medicine, 505 S. Preston St., Louisville, Louisville, Kentucky 40292, USA.; Department of Pharmacology and Toxicology, University of Louisville School of Medicine, 505 S. Hancock St., Louisville, Kentucky 40292, USA., Wise JP; Wise Laboratory of Environmental and Genetic Toxicology, Department of Pharmacology and Toxicology, University of Louisville School of Medicine, 505 S. Preston St., Louisville, Louisville, Kentucky 40292, USA.
Jazyk:	angličtina
Zdroj:	Toxicological sciences : an official journal of the Society of Toxicology [Toxicol Sci] 2021 Apr 27; Vol. 181 (1), pp. 115-124.
DOI:	10.1093/toxsci/kfab016
Abstrakt:	The northern Gulf of Mexico has a long history of polycyclic aromatic hydrocarbon (PAH) contamination from anthropogenic activities, natural oil seepages, and the 2010 Deepwater Horizon explosion and oil spill. The continental shelf of the same area is a known breeding ground for sperm whales (Physeter macrocephalus). To evaluate PAH-DNA damage, a biomarker for potential cancer risk, we compared skin biopsies collected from Gulf of Mexico sperm whales in 2012 with skin biopsies collected from sperm whales in areas of the Pacific Ocean in 1999-2001. All samples were obtained by crossbow and comprised both epidermis and subcutaneous blubber. To evaluate exposure, 7 carcinogenic PAHs were analyzed in lipids extracted from Pacific Ocean sperm whale blubber, pooled by sex, and location. To evaluate PAH-DNA damage, portions of all tissue samples were formalin-fixed, paraffin-embedded, sectioned, and examined for PAH-DNA adducts by immunohistochemistry (IHC) using an antiserum elicited against benzo[a]pyrene-modified DNA, which crossreacts with several high molecular weight carcinogenic PAHs bound to DNA. The IHC showed widespread epidermal nuclear localization of PAH-DNA adducts in the Gulf of Mexico whales (n = 15) but not in the Pacific Ocean whales (n = 4). A standard semiquantitative scoring system revealed significantly higher PAH-DNA adducts in the Gulf of Mexico whales compared to the whales from the Pacific Ocean study (p = .0002). (Published by Oxford University Press on behalf of the Society of Toxicology 2021. This work is written by US Government employees and is in the public domain in the US.)
Databáze:	MEDLINE
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