Distinct macrophage phenotypes and redox environment during the fin fold regenerative process in zebrafish.

Autor: Paredes LC; Department of Pathology, Federal University of Parana, Curitiba, Brazil., Luz RBDS; Department of Pathology, Federal University of Parana, Curitiba, Brazil., Tozzi ON; Department of Pathology, Federal University of Parana, Curitiba, Brazil., de Carvalho LÂSJ; Department of Pharmacology, Federal University of Parana, Curitiba, Brazil., Calado SLM; Department of Pharmacology, Federal University of Parana, Curitiba, Brazil., Padovani BN; Department of Immunology, Institute of Biomedical Sciences IV, University of São Paulo, São Paulo, Brazil., Fénero CIM; Department of Immunology, Institute of Biomedical Sciences IV, University of São Paulo, São Paulo, Brazil., do Amaral MA; Department of Immunology, Institute of Biomedical Sciences IV, University of São Paulo, São Paulo, Brazil., de Assis HCDS; Department of Pharmacology, Federal University of Parana, Curitiba, Brazil., Câmara NOS; Department of Immunology, Institute of Biomedical Sciences IV, University of São Paulo, São Paulo, Brazil.; Nephrology Division, Federal University of São Paulo, São Paulo, Brazil., Braga TT; Department of Pathology, Federal University of Parana, Curitiba, Brazil.; Graduate Program in Biosciences and Biotechnology, Instituto Carlos Chagas, Fiocruz-Parana, Curitiba, Brazil.
Jazyk: angličtina
Zdroj: Scandinavian journal of immunology [Scand J Immunol] 2021 Aug; Vol. 94 (2), pp. e13026. Date of Electronic Publication: 2021 Feb 22.
DOI: 10.1111/sji.13026
Abstrakt: In contrast to mammals, zebrafish (Danio rerio) has the ability to regenerate injured sites such as different tissues present in the fin. It is known that cells of the innate immune system play essential roles in regeneration; however, some aspects of the molecular mechanisms by which these cells orchestrate regeneration remain unknown. This study aimed to evaluate the infiltration dynamics of neutrophils and macrophages in the regenerative process of fin fold in regard to the influence of the redox environment and oxidative pathways. Fin fold amputation was performed on transgenic larvae for macrophage-expressed gene 1 (mpeg1), lysozyme (lyz), myeloperoxidase (mpo) and tumour necrosis factor alpha (TNFα) at 3 days post-fertilization, followed by confocal microscopy imaging and measurement of the activities of oxidant and antioxidant enzymes. We observed initially an increase in the number of neutrophils (lyz:DsRed+/mpx:GFP+) and then macrophages (mpeg1+) in the injury site followed by a decrease in neutrophils at 7 days post-amputation (dpa). Moreover, macrophages switch from a pro-inflammatory to an anti-inflammatory profile throughout the process, while the activity of superoxide dismutase (SOD) increased at 1 dpa and catalase (CAT) at 5 dpa. Higher levels of lipid peroxidation were also detected during regeneration. Despite oxidative stress, there is, therefore, an antioxidant response throughout the regeneration of the caudal fin. The present work can contribute to future studies on the development of cell therapies, achieving greater effectiveness in the treatment of diseases related to the formation of fibrotic tissue.
(© 2021 The Scandinavian Foundation for Immunology.)
Databáze: MEDLINE
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