Propofol suppresses cell viability, cell cycle progression and motility and induces cell apoptosis of ovarian cancer cells through suppressing MEK/ERK signaling via targeting circVPS13C/miR-145 axis.

Autor: Lu H; Department of Anesthesiology, Fujian Provincial Maternity and Children's Hospital, Affiliated Hospital of Fujian Medical University, No.18 daoshan Road, Fuzhou City, 350001, Fujian Province, China. huanlu2019@163.com., Zheng G; Department of Anesthesiology, Fujian Provincial Maternity and Children's Hospital, Affiliated Hospital of Fujian Medical University, No.18 daoshan Road, Fuzhou City, 350001, Fujian Province, China., Gao X; Department of Anesthesiology, Fujian Provincial Maternity and Children's Hospital, Affiliated Hospital of Fujian Medical University, No.18 daoshan Road, Fuzhou City, 350001, Fujian Province, China., Chen C; Department of Anesthesiology, Fujian Provincial Maternity and Children's Hospital, Affiliated Hospital of Fujian Medical University, No.18 daoshan Road, Fuzhou City, 350001, Fujian Province, China., Zhou M; Department of Anesthesiology, Fujian Provincial Maternity and Children's Hospital, Affiliated Hospital of Fujian Medical University, No.18 daoshan Road, Fuzhou City, 350001, Fujian Province, China., Zhang L; Department of Anesthesiology, Fujian Provincial Maternity and Children's Hospital, Affiliated Hospital of Fujian Medical University, No.18 daoshan Road, Fuzhou City, 350001, Fujian Province, China.
Jazyk: angličtina
Zdroj: Journal of ovarian research [J Ovarian Res] 2021 Feb 09; Vol. 14 (1), pp. 30. Date of Electronic Publication: 2021 Feb 09.
DOI: 10.1186/s13048-021-00775-3
Abstrakt: Background: Propofol is a kind of common intravenous anaesthetic agent that plays an anti-tumor role in a variety of cancers, including ovarian cancer. However, the working mechanism of Propofol in ovarian cancer needs further exploration.
Methods: The viability and metastasis of ovarian cancer cells were assessed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and transwell assays. Flow cytometry was used to evaluate the cell cycle and apoptosis. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to examine the abundance of circular RNA vacuolar protein sorting 13 homolog C (circVPS13C) and microRNA-145 (miR-145). The target relationship between miR-145 and circVPS13C was predicted by circinteractome database and verified by dual-luciferase reporter assay, RNA-binding protein immunoprecipitation (RIP) assay and RNA-pull down assay. Western blot assay was used to detect the levels of phosphorylated extracellular regulated MAP kinase (p-ERK), ERK, p-MAP kinse-ERK kinase (p-MEK) and MEK, in ovarian cancer cells.
Results: Propofol treatment suppressed the viability, cell cycle and motility and elevated the apoptosis rate of ovarian cancer cells. Propofol up-regulated miR-145 in a dose-dependent manner. Propofol exerted an anti-tumor role partly through up-regulating miR-145. MiR-145 was a direct target of circVPS13C. Propofol suppressed the progression of ovarian cancer through up-regulating miR-145 via suppressing circVPS13C. Propofol functioned through circVPS13C/miR-145/MEK/ERK signaling in ovarian cancer cells.
Conclusion: Propofol suppressed the proliferation, cell cycle, migration and invasion and induced the apoptosis of ovarian cancer cells through circVPS13C/miR-145/MEK/ERK signaling in vitro.
Databáze: MEDLINE
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