| Autor: |
Barmin RA; Skolkovo Institute of Science and Technology, 3 Nobelya Str., 121205 Moscow, Russia., Rudakovskaya PG; Skolkovo Institute of Science and Technology, 3 Nobelya Str., 121205 Moscow, Russia., Gusliakova OI; Remote Controlled Theranostic Systems Lab, Saratov State University, 83 Astrakhanskaya Str., 410012 Saratov, Russia., Sindeeva OA; Skolkovo Institute of Science and Technology, 3 Nobelya Str., 121205 Moscow, Russia.; Remote Controlled Theranostic Systems Lab, Saratov State University, 83 Astrakhanskaya Str., 410012 Saratov, Russia., Prikhozhdenko ES; Remote Controlled Theranostic Systems Lab, Saratov State University, 83 Astrakhanskaya Str., 410012 Saratov, Russia., Maksimova EA; Skolkovo Institute of Science and Technology, 3 Nobelya Str., 121205 Moscow, Russia., Obukhova EN; Skolkovo Institute of Science and Technology, 3 Nobelya Str., 121205 Moscow, Russia., Chernyshev VS; Skolkovo Institute of Science and Technology, 3 Nobelya Str., 121205 Moscow, Russia., Khlebtsov BN; Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, 410049 Saratov, Russia., Solovev AA; Department of Materials Science, Fudan University, Shanghai 200433, China., Sukhorukov GB; Skolkovo Institute of Science and Technology, 3 Nobelya Str., 121205 Moscow, Russia.; School of Engineering and Materials Science, Queen Mary University of London, Mile End Rd, London E1 4NS, UK., Gorin DA; Skolkovo Institute of Science and Technology, 3 Nobelya Str., 121205 Moscow, Russia. |
| Abstrakt: |
Microbubbles have already reached clinical practice as ultrasound contrast agents for angiography. However, modification of the bubbles' shell is needed to produce probes for ultrasound and multimodal (fluorescence/photoacoustic) imaging methods in combination with theranostics (diagnostics and therapeutics). In the present work, hybrid structures based on microbubbles with an air core and a shell composed of bovine serum albumin, albumin-coated gold nanoparticles, and clinically available photodynamic dyes (zinc phthalocyanine, indocyanine green) were shown to achieve multimodal imaging for potential applications in photodynamic therapy. Microbubbles with an average size of 1.5 ± 0.3 μm and concentration up to 1.2 × 10 9 microbubbles/mL were obtained and characterized. The introduction of the dye into the system reduced the solution's surface tension, leading to an increase in the concentration and stability of bubbles. The combination of gold nanoparticles and photodynamic dyes' influence on the fluorescent signal and probes' stability is described. The potential use of the obtained probes in biomedical applications was evaluated using fluorescence tomography, raster-scanning optoacoustic microscopy and ultrasound response measurements using a medical ultrasound device at the frequency of 33 MHz. The results demonstrate the impact of microbubbles' stabilization using gold nanoparticle/photodynamic dye hybrid structures to achieve probe applications in theranostics. |
