Resveratrol Promotes Hypertrophy in Wildtype Skeletal Muscle and Reduces Muscle Necrosis and Gene Expression of Inflammatory Markers in Mdx Mice.

Autor: Woodman KG; Murdoch Children's Research Institute, Royal Children's Hospital, Parkville, VIC 3052, Australia.; Faculty of Veterinary and Agricultural Science, The University of Melbourne, Parkville, VIC 3010, Australia.; Department of Genetics, Yale University, New Haven, CT 06510, USA., Coles CA; Murdoch Children's Research Institute, Royal Children's Hospital, Parkville, VIC 3052, Australia., Lamandé SR; Murdoch Children's Research Institute, Royal Children's Hospital, Parkville, VIC 3052, Australia.; Department of Paediatrics, The University of Melbourne, Parkville, VIC 3010, Australia., White JD; Faculty of Veterinary and Agricultural Science, The University of Melbourne, Parkville, VIC 3010, Australia.; Office of the Pro Vice Chancellor Research and Innovation, Charles Sturt University, Wagga, NSW 2678, Australia.
Jazyk: angličtina
Zdroj: Molecules (Basel, Switzerland) [Molecules] 2021 Feb 06; Vol. 26 (4). Date of Electronic Publication: 2021 Feb 06.
DOI: 10.3390/molecules26040853
Abstrakt: Duchenne muscular dystrophy (DMD) is a progressive fatal neuromuscular disorder with no cure. Therapies to restore dystrophin deficiency have been approved in some jurisdictions but long-term effectiveness is yet to be established. There is a need to develop alternative strategies to treat DMD. Resveratrol is a nutraceutical with anti-inflammatory properties. Previous studies have shown high doses (100-400 mg/kg bodyweight/day) benefit mdx mice. We treated 4-week-old mdx and wildtype mice with a lower dose of resveratrol (5 mg/kg bodyweight/day) for 15 weeks. Voluntary exercise was used to test if a lower dosage than previously tested could reduce exercise-induced damage where a greater inflammatory infiltrate is present. We found resveratrol promoted skeletal muscle hypertrophy in wildtype mice. In dystrophic muscle, resveratrol reduced exercise-induced muscle necrosis. Gene expression of immune cell markers, CD86 and CD163 were reduced; however, signalling targets associated with resveratrol's mechanism of action including Sirt1 and NF-κB were unchanged. In conclusion, a lower dose of resveratrol compared to the dosage used by other studies reduced necrosis and gene expression of inflammatory cell markers in dystrophic muscle suggesting it as a therapeutic candidate for treating DMD.
Databáze: MEDLINE
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