Chromosomal assembly of the nuclear genome of the endosymbiont-bearing trypanosomatid Angomonas deanei.

Autor: Davey JW; Department of Biology, University of York, York YO10 5DD, UK., Catta-Preta CMC; Department of Biology, University of York, York YO10 5DD, UK.; York Biomedical Research Institute, University of York, York YO10 5DD, UK.; Medicinal Chemistry Center (CQMED)/Structural Genomics Consortium, Universidade Estadual de Campinas, Campinas, São Paulo 13083-886, Brazil., James S; Department of Biology, University of York, York YO10 5DD, UK., Forrester S; Department of Biology, University of York, York YO10 5DD, UK., Motta MCM; Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Departamento de Biologia Celular e Parasitologia, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.; Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Bioimagens, Rio de Janeiro, RJ, Brazil., Ashton PD; Department of Biology, University of York, York YO10 5DD, UK., Mottram JC; Department of Biology, University of York, York YO10 5DD, UK.; York Biomedical Research Institute, University of York, York YO10 5DD, UK.
Jazyk: angličtina
Zdroj: G3 (Bethesda, Md.) [G3 (Bethesda)] 2021 Jan 18; Vol. 11 (1).
DOI: 10.1093/g3journal/jkaa018
Abstrakt: Angomonas deanei is an endosymbiont-bearing trypanosomatid with several highly fragmented genome assemblies and unknown chromosome number. We present an assembly of the A. deanei nuclear genome based on Oxford Nanopore sequence that resolves into 29 complete or close-to-complete chromosomes. The assembly has several previously unknown special features; it has a supernumerary chromosome, a chromosome with a 340-kb inversion, and there is a translocation between two chromosomes. We also present an updated annotation of the chromosomal genome with 10,365 protein-coding genes, 59 transfer RNAs, 26 ribosomal RNAs, and 62 noncoding RNAs.
(© The Author(s) 2020. Published by Oxford University Press on behalf of Genetics Society of America.)
Databáze: MEDLINE
Externí odkaz: