An effective polymeric nanocarrier that allows for active targeting and selective drug delivery in cell coculture systems.

Autor: Cano-Cortes MV; GENYO, Centre for Genomics and Oncological Research, Pfizer/University of Granada/Andalusian Regional Government, PTS Granada, Avda. Ilustración 114, 18016 Granada, Spain. rmsanchez@go.ugr.es juandiaz@go.ugr.es and Department of Medicinal & Organic Chemistry and Excellence Research Unit of 'Chemistry applied to Biomedicine and the Environment', Faculty of Pharmacy, University of Granada, Campus de Cartuja s/n, 18071, Granada, Spain and Biosanitary Research Institute of Granada (ibs.GRANADA), University Hospitals of Granada-University of Granada, Granada, 18071, Spain., Altea-Manzano P; GENYO, Centre for Genomics and Oncological Research, Pfizer/University of Granada/Andalusian Regional Government, PTS Granada, Avda. Ilustración 114, 18016 Granada, Spain. rmsanchez@go.ugr.es juandiaz@go.ugr.es and Laboratory of Cellular Metabolism and Metabolic Regulation, VIB-KU Leuven Center for Cancer Biology, Campus GasthuisberǵHerestraat 49, 3000 Leuven, Belgium., Laz-Ruiz JA; GENYO, Centre for Genomics and Oncological Research, Pfizer/University of Granada/Andalusian Regional Government, PTS Granada, Avda. Ilustración 114, 18016 Granada, Spain. rmsanchez@go.ugr.es juandiaz@go.ugr.es and Department of Medicinal & Organic Chemistry and Excellence Research Unit of 'Chemistry applied to Biomedicine and the Environment', Faculty of Pharmacy, University of Granada, Campus de Cartuja s/n, 18071, Granada, Spain and Biosanitary Research Institute of Granada (ibs.GRANADA), University Hospitals of Granada-University of Granada, Granada, 18071, Spain., Unciti-Broceta JD; Nanogetic S.L. Avda de la Innovacion 1, PTS, Edificio BIC, 18016 - Granada, Spain., Lopez-Delgado FJ; GENYO, Centre for Genomics and Oncological Research, Pfizer/University of Granada/Andalusian Regional Government, PTS Granada, Avda. Ilustración 114, 18016 Granada, Spain. rmsanchez@go.ugr.es juandiaz@go.ugr.es and DestiNA Genomica S.L. PTS Granada, Avenida de la Innovación 1, Edificio BIC, 18016, Granada, Spain., Espejo-Roman JM; GENYO, Centre for Genomics and Oncological Research, Pfizer/University of Granada/Andalusian Regional Government, PTS Granada, Avda. Ilustración 114, 18016 Granada, Spain. rmsanchez@go.ugr.es juandiaz@go.ugr.es and Department of Medicinal & Organic Chemistry and Excellence Research Unit of 'Chemistry applied to Biomedicine and the Environment', Faculty of Pharmacy, University of Granada, Campus de Cartuja s/n, 18071, Granada, Spain and Biosanitary Research Institute of Granada (ibs.GRANADA), University Hospitals of Granada-University of Granada, Granada, 18071, Spain., Diaz-Mochon JJ; GENYO, Centre for Genomics and Oncological Research, Pfizer/University of Granada/Andalusian Regional Government, PTS Granada, Avda. Ilustración 114, 18016 Granada, Spain. rmsanchez@go.ugr.es juandiaz@go.ugr.es and Department of Medicinal & Organic Chemistry and Excellence Research Unit of 'Chemistry applied to Biomedicine and the Environment', Faculty of Pharmacy, University of Granada, Campus de Cartuja s/n, 18071, Granada, Spain and Biosanitary Research Institute of Granada (ibs.GRANADA), University Hospitals of Granada-University of Granada, Granada, 18071, Spain., Sanchez-Martin RM; GENYO, Centre for Genomics and Oncological Research, Pfizer/University of Granada/Andalusian Regional Government, PTS Granada, Avda. Ilustración 114, 18016 Granada, Spain. rmsanchez@go.ugr.es juandiaz@go.ugr.es and Department of Medicinal & Organic Chemistry and Excellence Research Unit of 'Chemistry applied to Biomedicine and the Environment', Faculty of Pharmacy, University of Granada, Campus de Cartuja s/n, 18071, Granada, Spain and Biosanitary Research Institute of Granada (ibs.GRANADA), University Hospitals of Granada-University of Granada, Granada, 18071, Spain.
Jazyk: angličtina
Zdroj: Nanoscale [Nanoscale] 2021 Feb 18; Vol. 13 (6), pp. 3500-3511.
DOI: 10.1039/d0nr07145e
Abstrakt: In this manuscript, we report the development of a versatile, robust, and stable targeting nanocarrier for active delivery. This nanocarrier is based on bifunctionalized polymeric nanoparticles conjugated to a monoclonal antibody that allows for active targeting of either (i) a fluorophore for tracking or (ii) a drug for monitoring specific cell responses. This nanodevice can efficiently discriminate between cells in coculture based on the expression levels of cell surface receptors. As a proof of concept, we have demonstrated efficient delivery using a broadly established cell surface receptor as the target, the epidermal growth factor receptor (EGFR), which is overexpressed in several types of cancers. Additionally, a second validation of this nanodevice was successfully carried out using another cell surface receptor as the target, the cluster of differentiation 147 (CD147). Our results suggest that this versatile nanocarrier can be expanded to other cell receptors and bioactive cargoes, offering remarkable discrimination efficiency between cells with different expression levels of a specific marker. This work supports the ability of nanoplatforms to boost and improve the progress towards personalized medicine.
Databáze: MEDLINE