Veratrum Alkaloid Determination in Four Cases of Veratrum Aqua Poisonings.

Autor:	Melnik EV; A.P. Arzamastsev Department of Pharmaceutical and Toxicological Chemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University), 2-4 Bolshaya Pirogovskaya Street, Moscow 119991, Russian Federation., Belova MV; A.P. Arzamastsev Department of Pharmaceutical and Toxicological Chemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University), 2-4 Bolshaya Pirogovskaya Street, Moscow 119991, Russian Federation.; Department of Acute Poisonings and Somatopsychiatric Disorders, N.V. Sklifosovsky Research Institute for Emergency Medicine, 3 Bolshaya Sukharevskaya Square, Moscow 129090, Russian Federation., Potskhveriya MM; Department of Acute Poisonings and Somatopsychiatric Disorders, N.V. Sklifosovsky Research Institute for Emergency Medicine, 3 Bolshaya Sukharevskaya Square, Moscow 129090, Russian Federation., Simonova AY; Department of Acute Poisonings and Somatopsychiatric Disorders, N.V. Sklifosovsky Research Institute for Emergency Medicine, 3 Bolshaya Sukharevskaya Square, Moscow 129090, Russian Federation., Tyurin IA; Department of Acute Poisonings and Somatopsychiatric Disorders, N.V. Sklifosovsky Research Institute for Emergency Medicine, 3 Bolshaya Sukharevskaya Square, Moscow 129090, Russian Federation., Ramenskaya GV; A.P. Arzamastsev Department of Pharmaceutical and Toxicological Chemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University), 2-4 Bolshaya Pirogovskaya Street, Moscow 119991, Russian Federation.
Jazyk:	angličtina
Zdroj:	Journal of analytical toxicology [J Anal Toxicol] 2022 Feb 14; Vol. 46 (1), pp. e42-e47.
DOI:	10.1093/jat/bkab019
Abstrakt:	Veratrum poisonings are described in the toxicology literature as multiple Veratrum species grow in different parts of the Northern Hemisphere and are occasionally ingested by mistake. Veratrum toxicity is attributed to the steroidal alkaloids contained in all parts of the plant. In Russia, Veratrum poisonings are more common since there is an over-the-counter Veratrum lobelianum-based tincture, Veratrum Aqua (VA), which is topically used for the treatment of lice infestation. Despite its toxicity, VA is misused in traditional medicine as a remedy for alcohol use disorder. We describe four cases of VA poisoning that occurred in Moscow, Russia. Three main V. lobelianum alkaloids (jervine, protoveratrine A (proA) and protoveratrine B) were determined in patient plasma and urine samples using liquid chromatography-tandem mass spectrometry (LC-MS-MS). Here, we describe a novel validated LC-MS-MS method for jervine and proA quantification. A simple and rapid liquid-liquid extraction with methyl tert-butyl ether was utilized for analyte extraction. Chromatographic separation was achieved using a Poroshell 120 EC-C18 column, and the total run time was 14 min. The lower limit of quantification was 0.1 ng/mL for jervine and proA in both plasma and urine. Biological samples were obtained upon hospital admission and during treatment, thus enabling to get a better understanding of the alkaloid elimination profile. Upon admission, plasma concentrations of jervine (concentration range: 0.10-5.01 ng/mL) prevailed over proA (concentration range: 0-0.67 ng/mL). At this time, proA already reached maximum concentrations in urine (concentration range: 0.15-37.70 ng/mL). Maximum concentrations of jervine in urine were observed 24 h after admission (concentration range: 0.10-9.55 ng/mL). In all cases, plasma concentrations of Veratrum alkaloids correlated with condition severity. Since none of the patients confirmed VA intake, instrumental analysis was the basis for the definitive diagnosis of VA poisoning. (© The Author(s) 2021. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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