Evaluation of the gastroprotective effects of Chihuahua propolis on indomethacin- induced gastric ulcers in mouse.

Autor: Ruiz-Hurtado PA; Laboratorio de Toxicología de Productos Naturales, Departamento de Farmacia, IPN, Escuela Nacional de Ciencias Biológicas, Av. Wilfrido Massieu, Gustavo A. Madero, 07738 Ciudad de México, México; Laboratorio de Investigación Biomédica en Productos Naturales, Carrera de Medicina, UNAM, FES-Iztacala, Avenida de los Barrios Número 1, Colonia Los Reyes Iztacala, 54090 Tlalnepantla, Estado de México, México. Electronic address: alonsoruiz55@gmail.com., Garduño-Siciliano L; Laboratorio de Toxicología de Productos Naturales, Departamento de Farmacia, IPN, Escuela Nacional de Ciencias Biológicas, Av. Wilfrido Massieu, Gustavo A. Madero, 07738 Ciudad de México, México. Electronic address: lsicilia@hotmail.com., Dominguez-Verano P; Laboratorio de Investigación Biomédica en Productos Naturales, Carrera de Medicina, UNAM, FES-Iztacala, Avenida de los Barrios Número 1, Colonia Los Reyes Iztacala, 54090 Tlalnepantla, Estado de México, México. Electronic address: pilardomver@hotmail.com., Martinez-Galero E; Laboratorio de Toxicología de la Reproducción-Teratogénesis, Departamento de Farmacia, IPN, Escuela Nacional de Ciencias Biológicas, Av. Wilfrido Massieu, Gustavo A. Madero, 07738 Ciudad de México, México. Electronic address: emartingal@gmail.com., Canales-Martinez MM; Laboratorio de Farmacognosia, UBIPRO, UNAM, FES-Iztacala, Avenida de los Barrios Número 1, Colonia Los Reyes Iztacala, 54090 Tlalnepantla, Estado de México, México. Electronic address: dra.margaritacanales@gmail.com., Rodriguez-Monroy MA; Laboratorio de Investigación Biomédica en Productos Naturales, Carrera de Medicina, UNAM, FES-Iztacala, Avenida de los Barrios Número 1, Colonia Los Reyes Iztacala, 54090 Tlalnepantla, Estado de México, México. Electronic address: dr.marcorodriguezmonroy@gmail.com.
Jazyk: angličtina
Zdroj: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2021 May; Vol. 137, pp. 111345. Date of Electronic Publication: 2021 Feb 05.
DOI: 10.1016/j.biopha.2021.111345
Abstrakt: The aim of this work was to evaluate the gastroprotective activity of a Mexican propolis on indomethacin-induced gastric ulcers in a mouse model. The following contents of the ethanolic extract of propolis of Chihuahua (EEPCh) were determined: antioxidant activity (SA 50 ), total phenolic content (TPC), total flavonoid content (TFC), and chemical composition by HPLC-DAD and HPLC-MS, as well as acute toxicity by OECD Guideline 423. Gastric lesions were induced by intragastric indomethacin treatment in male ICR mice. As the positive control, omeprazole was administered, and three doses of EEPCh were evaluated (50, 150 and 300 mg/kg). Gastric mucosal injury, histological changes and mucosal content were evaluated by means of H&E and PAS staining. For homogenized gastric tissues, the following were evaluated: TBARS, MPO, and PGE 2 levels; SOD and GPx antioxidant enzymatic activity; and the concentrations of the proinflammatory cytokines, TNF-α, IL-1β and IL-6. EEPCh had a significant SA 50 of 41.55 µg/mL. The TPC of EEPCh was 860 mg GAE/g, and its TFC was 49.58 mg QE/g. Different phenolic compounds were identified in the extract and were not toxic. The EEPCh doses decreased mucosal damage and histological injuries, maintained the mucosal content and reduced the TBARS, MPO and concentrations of proinflammatory cytokines in gastric ulcer tissues. The 150 and 300 mg/kg doses increased the SOD activity and maintained the PGE 2 content. Only the 300 mg/kg dose increased the GPx activity. The results of this study suggest that EEPCh displays gastroprotective effects by means of its antioxidant activity and anti-inflammatory effects and promotes ulcer protection through the maintenance of mucosal content and PGE 2 levels.
(Copyright © 2021 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
Databáze: MEDLINE
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