Evaluation of active matrix metalloproteinase-8 (aMMP-8) chair-side test as a diagnostic biomarker in the staging of periodontal diseases.

Autor: Öztürk VÖ; Department of Periodontology, School of Dentistry, Aydın Adnan Menderes University, Aydın, Turkey. Electronic address: v.ozgenozturk@gmail.com., Emingil G; Department of Periodontology, School of Dentistry, Ege University, İzmir, Turkey., Umeizudike K; Department of Preventive Dentistry, Faculty of Dental Sciences, College of Medicine, University of Lagos, Idi-araba, Lagos, Nigeria., Tervahartiala T; Department of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki University Hospital, Institute of Dentistry, Helsinki, Finland., Gieselmann DR; Institute of Molecular Dentistry and Dentognostics GmbH, Solingen and Jena, Germany., Maier K; Institute of Molecular Dentistry and Dentognostics GmbH, Solingen and Jena, Germany., Köse T; Department of Biostatistics and Medical Informatics, School of Medicine, Ege University, Izmir, Turkey., Sorsa T; Department of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki University Hospital, Institute of Dentistry, Helsinki, Finland; Division of Oral Diseases, Department of Dental Medicine, Karolinksa Institute, Huddinge, Sweden., Alassiri S; Department of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki University Hospital, Institute of Dentistry, Helsinki, Finland.
Jazyk: angličtina
Zdroj: Archives of oral biology [Arch Oral Biol] 2021 Apr; Vol. 124, pp. 104955. Date of Electronic Publication: 2021 Jan 20.
DOI: 10.1016/j.archoralbio.2020.104955
Abstrakt: Objective: There is a need for a reliable complementary diagnostic tool that ideally helps to screen, differentiate sites, activities of and predict future periodontal tissue destruction. The purpose of this cross-sectional study was to investigate the screening and prevention potential of the chair-side/point-of-care (PoC) diagnostic test of salivary active matrix metalloproteinase-8 (aMMP-8) levels at different stages of periodontal disease and periodontal health.
Material & Methods: 80 individuals were included in this study; 18 with periodontitis stage 3 (P-Stage III), 19 with periodontitis stage-4 (P-Stage IV), 21 with gingivitis, and 22 with clinically healthy periodontium (H). The aMMP-8 levels in GCF and saliva were analyzed by chairside point-of-care aMMP-8 lateral flow immunotest and also by a time-resolved immunofluorescence assay (IFMA).
Results: The sensitivity of the chair-side/PoC test was 83.9 % while specificity was 79.2 %. The aMMP-8 IFMA levels in GCF were significantly higher in P-Stage IV group than P-Stage III, gingivitis and healthy groups (p = 0.01, p = 0.001, p = 0.00, respectively). Moreover, P-Stage III and gingivitis groups had significantly higher aMMP-8 IFMA levels than the healthy group (p < 0.05).
Conclusion: The aMMP-8 chair-side test showed promising results in its ability to recognize and predict the inflammatory status even at the very initial/early stages. aMMP-8 chair-side test could be a valuable adjunctive diagnostic and preventive tool to conventional clinical methods in detecting periodontal disease.
(Copyright © 2020. Published by Elsevier Ltd.)
Databáze: MEDLINE
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