Circular RNA circANKS1B acts as a sponge for miR-152-3p and promotes prostate cancer progression by upregulating TGF-α expression.

Autor: Tao LJ; Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.; Institute of Urology, Anhui Medical University, Hefei, Anhui, China.; Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei, Anhui, China., Pan XY; Department of Ophthalmology, The Second People's Hospital of Wuhu, Wuhu, Anhui, China., Wang JW; Department of Urology, The Second People's Hospital of Wuhu, Wuhu, Anhui, China., Zhang L; Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.; Institute of Urology, Anhui Medical University, Hefei, Anhui, China.; Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei, Anhui, China., Tao LS; Department of Urology, The Second People's Hospital of Wuhu, Wuhu, Anhui, China., Liang CZ; Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.; Institute of Urology, Anhui Medical University, Hefei, Anhui, China.; Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei, Anhui, China.
Jazyk: angličtina
Zdroj: The Prostate [Prostate] 2021 Apr; Vol. 81 (5), pp. 271-278. Date of Electronic Publication: 2021 Feb 08.
DOI: 10.1002/pros.24102
Abstrakt: Background: A growing number of studies indicate that circular RNAs (circRNAs) play critical roles in human diseases, and show great potential as biomarkers and therapeutic targets. This study aimed to investigate the expression and function of circANKS1B in prostate cancer (PC).
Methods: The expression of circANKS1B and miR-152-3p was analyzed by real-time quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Cell migration and invasion were measured using a transwell assay. The interaction between circANKS1B and miR-152-3p was confirmed by a dual-luciferase reporter gene assay. Rescue experiments were conducted to determine whether circANKS1B regulated the invasion of PC cells via the circANKS1B-miR-152-3p-TGF-α pathway.
Results: The expression of circANKS1B was markedly upregulated in both PC cells and tissues. Moreover, high circANKS1B expression was associated with poor prognosis in PC patients. Dual-luciferase reporter assay indicated that circANKS1B directly bound to miR-152-3p. Furthermore, circANKS1B negatively regulated miR-152-3p expression. Knockdown of circANKS1B markedly suppressed cell migration and invasion and TGF-α expression in PC cells, whereas the effects of circANKS1B silencing were reversed by miR-152-3p deficiency. In addition, the impact of miR-152-3p silencing on invasion of circANKS1B-deficient PC cells was also abrogated by TGF-α deficiency. Overall, circANKS1B acts as a sponge for miR-152-3p to promote PC progression by upregulating TGF-α expression.
Conclusion: Our findings reveal that circANKS1B may be a potential prognostic biomarker and therapeutic target for PC.
(© 2021 Wiley Periodicals LLC.)
Databáze: MEDLINE
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