| Autor: |
Rettenmaier R; Technical University of Munich, Chair of Microbiology, Emil-Ramann-Str. 4, 85354 Freising, Germany., Kowollik ML; Technical University of Munich, Chair of Microbiology, Emil-Ramann-Str. 4, 85354 Freising, Germany., Klingl A; LMU Munich, Plant Development & Electron Microscopy, Biocenter LMU Munich, Großhadernerstr. 2-4, 82152 Planegg-Martinsried, Germany., Liebl W; Technical University of Munich, Chair of Microbiology, Emil-Ramann-Str. 4, 85354 Freising, Germany., Zverlov V; Institute of Molecular Genetics of National Research Centre «Kurchatov Institute», Kurchatov Sq. 2, 123182 Moscow, Russia.; Technical University of Munich, Chair of Microbiology, Emil-Ramann-Str. 4, 85354 Freising, Germany. |
| Jazyk: |
angličtina |
| Zdroj: |
International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2019 Jun; Vol. 71 (3). Date of Electronic Publication: 2021 Feb 08. |
| DOI: |
10.1099/ijsem.0.004692 |
| Abstrakt: |
An anaerobic bacterial strain, designated MA18 T , was isolated from a laboratory-scale biogas fermenter fed with maize silage. Cells stained Gram-negative and performed Gram-negative in the KOH test. The peptidoglycan type was found to be A1y-meso-Dpm direct. The major cellular fatty acids were C 14 : 0 iso, C 15 : 0 iso, anteiso and iso DMA as well as a C 16 unidentified fatty acid. Oxidase and catalase activities were absent. Cells were slightly curved rods, motile, formed spores and measured approximately 0.35 µm in diameter and 3.0-5.0 µm in length. When cultivated on GS2 agar with cellobiose, round, arched, shiny and slightly yellow-pigmented colonies were formed. The isolate was mesophilic to moderately thermophilic with a growth optimum between 40 and 48 °C. Furthermore, neutral pH values were preferred and up to 1.2 % (w/v) NaCl supplemented to the GS2 medium was tolerated. Producing mainly acetate and ethanol, MA18 T fermented arabinose, cellobiose, crystalline and amorphous cellulose, ribose, and xylan. The genome of MA18 T consists of 4 817 678 bp with a G+C content of 33.16 mol%. In the annotated protein sequences, cellulosomal components were detected. Phylogenetically, MA18 T is most closely related to Ruminiclostridium sufflavum DSM 19573 T (76.88 % average nucleotide identity of the whole genome sequence; 97.23 % 16S rRNA gene sequence similarity) and can be clustered into one clade with other species of the genus Ruminiclostridium , family Oscillospiraceae , class Clostridia . Based on morphological, physiological and genetic characteristics, this strain represents a novel species in the genus Ruminiclostridium . Therefore, the name Ruminiclostridium herbifermentans sp. nov. is proposed. The type strain is MA18 T (=DSM 109966 T =JCM 39124 T ). |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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