p97 and p47 function in membrane tethering in cooperation with FTCD during mitotic Golgi reassembly.
Autor: | Kaneko Y; Department of Molecular Cell Biology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan., Shimoda K; Department of Molecular Cell Biology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan., Ayala R; Section of Structural Biology, Department of Infectious Diseases, Imperial College London, London, UK., Goto Y; Department of Molecular Cell Biology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan., Panico S; Section of Structural Biology, Department of Infectious Diseases, Imperial College London, London, UK., Zhang X; Section of Structural Biology, Department of Infectious Diseases, Imperial College London, London, UK., Kondo H; Department of Molecular Cell Biology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. |
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Jazyk: | angličtina |
Zdroj: | The EMBO journal [EMBO J] 2021 May 03; Vol. 40 (9), pp. e105853. Date of Electronic Publication: 2021 Feb 08. |
DOI: | 10.15252/embj.2020105853 |
Abstrakt: | p97ATPase-mediated membrane fusion is required for the biogenesis of the Golgi complex. p97 and its cofactor p47 function in soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptor (SNARE) priming, but the tethering complex for p97/p47-mediated membrane fusion remains unknown. In this study, we identified formiminotransferase cyclodeaminase (FTCD) as a novel p47-binding protein. FTCD mainly localizes to the Golgi complex and binds to either p47 or p97 via its association with their polyglutamate motifs. FTCD functions in p97/p47-mediated Golgi reassembly at mitosis in vivo and in vitro via its binding to p47 and to p97. We also showed that FTCD, p47, and p97 form a big FTCD-p97/p47-FTCD tethering complex. In vivo tethering assay revealed that FTCD that was designed to localize to mitochondria caused mitochondria aggregation at mitosis by forming a complex with endogenous p97 and p47, which support a role for FTCD in tethering biological membranes in cooperation with the p97/p47 complex. Therefore, FTCD is thought to act as a tethering factor by forming the FTCD-p97/p47-FTCD complex in p97/p47-mediated Golgi membrane fusion. (© 2021 The Authors.) |
Databáze: | MEDLINE |
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