Hemocytometric characteristics of COVID-19 patients with and without cytokine storm syndrome on the sysmex XN-10 hematology analyzer.

Autor:	Martens RJH; Department of Clinical Chemistry and Hematology, Zuyderland Medical Center, Heerlen & Sittard, The Netherlands., van Adrichem AJ; Department of Clinical Chemistry and Hematology, Zuyderland Medical Center, Heerlen & Sittard, The Netherlands., Mattheij NJA; Department of Clinical Chemistry and Hematology, Zuyderland Medical Center, Heerlen & Sittard, The Netherlands., Brouwer CG; Department of Internal Medicine, Zuyderland Medical Center, Heerlen & Sittard, The Netherlands., van Twist DJL; Department of Internal Medicine, Zuyderland Medical Center, Heerlen & Sittard, The Netherlands., Broerse JJCR; Department of Pulmonology, Zuyderland Medical Center, Heerlen & Sittard, The Netherlands., Magro-Checa C; Department of Rheumatology, Zuyderland Medical Center, Heerlen & Sittard, The Netherlands., van Dongen CMP; Department of Rheumatology, Zuyderland Medical Center, Heerlen & Sittard, The Netherlands., Mostard RLM; Department of Pulmonology, Zuyderland Medical Center, Heerlen & Sittard, The Netherlands., Ramiro S; Department of Rheumatology, Zuyderland Medical Center, Heerlen & Sittard, The Netherlands.; Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands., Landewé RBM; Department of Rheumatology, Zuyderland Medical Center, Heerlen & Sittard, The Netherlands.; Department of Clinical Immunology and Rheumatology, Amsterdam University Medical Center, Amsterdam, The Netherlands., Leers MPG; Department of Clinical Chemistry and Hematology, Zuyderland Medical Center, Heerlen & Sittard, The Netherlands.
Jazyk:	angličtina
Zdroj:	Clinical chemistry and laboratory medicine [Clin Chem Lab Med] 2020 Dec 09; Vol. 59 (4), pp. 783-793. Date of Electronic Publication: 2020 Dec 09 (Print Publication: 2021).
DOI:	10.1515/cclm-2020-1529
Abstrakt:	Objectives: COVID-19 is an ongoing global pandemic. There is an urgent need for identification and understanding of clinical and laboratory parameters related to progression towards a severe and fatal form of this illness, often preceded by a so-called cytokine-storm syndrome (CSS). Therefore, we explored the hemocytometric characteristics of COVID-19 patients in relation to the deteriorating clinical condition CSS, using the Sysmex XN-10 hematology analyzer. Methods: From March 1st till May 16th, 2020, all patients admitted to our hospital with respiratory complaints and suspected for COVID-19 were included (n=1,140 of whom n=533 COVID-19 positive). The hemocytometric parameters of immunocompetent cells in peripheral blood (neutrophils [NE], lymphocytes [LY] and monocytes [MO]) obtained upon admission to the emergency department (ED) of COVID-19 positive patients were compared with those of the COVID-19 negative ones. Moreover, patients with CSS (n=169) were compared with COVID-19 positive patients without CSS, as well as with COVID-19 negative ones. Results: In addition to a significant reduction in leukocytes, thrombocytes and absolute neutrophils, it appeared that lymphocytes-forward scatter (LY-FSC), and reactive lymphocytes (RE-LYMPHO)/leukocytes were higher in COVID-19-positive than negative patients. At the moment of presentation, COVID-19 positive patients with CSS had different neutrophils-side fluorescence (NE-SFL), neutrophils-forward scatter (NE-FSC), LY-FSC, RE-LYMPHO/lymphocytes, antibody-synthesizing (AS)-LYMPHOs, high fluorescence lymphocytes (HFLC), MO-SSC, MO-SFL, and Reactive (RE)-MONOs. Finally, absolute eosinophils, basophils, lymphocytes, monocytes and MO-FSC were lower in patients with CSS. Conclusions: Hemocytometric parameters indicative of changes in immunocompetent peripheral blood cells and measured at admission to the ED were associated with COVID-19 with and without CSS. (© 2020 Remy J. H. Martens et al., published by De Gruyter, Berlin/Boston.)
Databáze:	MEDLINE
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