An accurate, high-speed, portable bifunctional electrical detector for COVID-19.

Autor: Ke G; Institute of Polymer Optoelectronic Materials and Devices, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou, 510640 China.; Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871 China., Su D; Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871 China., Li Y; Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871 China., Zhao Y; Department of Energy and Resources Engineering, College of Engineering, Peking University, Beijing, 100871 China., Wang H; Department of Energy and Resources Engineering, College of Engineering, Peking University, Beijing, 100871 China., Liu W; Qingdao Shuojing Biological Technology Co., Ltd., Qingdao, 266112 China., Li M; Department of Pathology, Beijing Ditan Hospital Capital Medical University, Beijing, 100015 China., Yang Z; Qingdao Shuojing Biological Technology Co., Ltd., Qingdao, 266112 China., Xiao F; Institute of Digital Economy Industry, Hangzhou, 310015 China., Yuan Y; Beijing Sylincom Technology Co., Ltd., Beijing, 100081 China., Huang F; Institute of Polymer Optoelectronic Materials and Devices, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou, 510640 China., Mo F; Department of Energy and Resources Engineering, College of Engineering, Peking University, Beijing, 100871 China., Wang P; Department of Pathology, Beijing Ditan Hospital Capital Medical University, Beijing, 100015 China., Guo X; Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871 China.
Jazyk: angličtina
Zdroj: Science China materials [Sci China Mater] 2021; Vol. 64 (3), pp. 739-747. Date of Electronic Publication: 2020 Dec 29.
DOI: 10.1007/s40843-020-1577-y
Abstrakt: Coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2, has rapidly spread and caused a severe global pandemic. Because no specific drugs are available for COVID-19 and few vaccines are available for SARS-CoV-2, accurate and rapid diagnosis of COVID-19 has been the most crucial measure to control this pandemic. Here, we developed a portable bifunctional electrical detector based on graphene fieldeffect transistors for SARS-CoV-2 through either nucleic acid hybridization or antigen-antibody protein interaction, with ultra-low limits of detection of ~0.1 and ~1 fg mL -1 in phosphate buffer saline, respectively. We validated our method by assessment of RNA extracts from the oropharyngeal swabs of ten COVID-19 patients and eight healthy subjects, and the IgM/IgG antibodies from serum specimens of six COVID-19 patients and three healthy subjects. Here we show that the diagnostic results are in excellent agreement with the findings of polymerase chain reaction-based optical methods; they also exhibit rapid detection speed (~10 min for nucleic acid detection and ~5 min for immunoassay). Therefore, our assay provides an efficient, accurate tool for high-throughput point-of-care testing.
Electronic Supplementary Material: Supplementary material is available in the online version of this article at 10.1007/s40843-020-1577-y.
(© Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020.)
Databáze: MEDLINE
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