Responses of the Necrotrophic Fungus Alternaria brassisicola to the Indolic Phytoalexin Brassinin.

Autor:	N'Guyen GQ; UNIV Angers, Institut Agro, INRAE, IRHS, SFR 4207 QuaSaV, Angers, France., Raulo R; Institut Charles Viollette - EA 7394, Université de Lille, INRA, ISA, Université d'Artois, Université du Littoral Côte d'Opale, Lille, France., Porquier A; BIOGER - INRA UR1290, Thiverval-Grignon, France., Iacomi B; USAMV, Bucharest, Romania., Pelletier S; UNIV Angers, Institut Agro, INRAE, IRHS, SFR 4207 QuaSaV, Angers, France., Renou JP; UNIV Angers, Institut Agro, INRAE, IRHS, SFR 4207 QuaSaV, Angers, France., Bataillé-Simoneau N; UNIV Angers, Institut Agro, INRAE, IRHS, SFR 4207 QuaSaV, Angers, France., Campion C; UNIV Angers, Institut Agro, INRAE, IRHS, SFR 4207 QuaSaV, Angers, France., Hamon B; UNIV Angers, Institut Agro, INRAE, IRHS, SFR 4207 QuaSaV, Angers, France., Kwasiborski A; UNIV Angers, Institut Agro, INRAE, IRHS, SFR 4207 QuaSaV, Angers, France., Colou J; UNIV Angers, Institut Agro, INRAE, IRHS, SFR 4207 QuaSaV, Angers, France., Benamar A; UNIV Angers, Institut Agro, INRAE, IRHS, SFR 4207 QuaSaV, Angers, France., Hudhomme P; CNRS, Moltech Anjou, UNIV Angers, Angers, France., Macherel D; UNIV Angers, Institut Agro, INRAE, IRHS, SFR 4207 QuaSaV, Angers, France., Simoneau P; UNIV Angers, Institut Agro, INRAE, IRHS, SFR 4207 QuaSaV, Angers, France., Guillemette T; UNIV Angers, Institut Agro, INRAE, IRHS, SFR 4207 QuaSaV, Angers, France.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2021 Jan 14; Vol. 11, pp. 611643. Date of Electronic Publication: 2021 Jan 14 (Print Publication: 2020).
DOI:	10.3389/fpls.2020.611643
Abstrakt:	Alternaria brassicicola causes black spot disease in Brassicaceae . During host infection, this necrotrophic fungus is exposed to various antimicrobial compounds, such as the phytoalexin brassinin which is produced by many cultivated Brassica species. To investigate the cellular mechanisms by which this compound causes toxicity and the corresponding fungal adaptive strategies, we first analyzed fungal transcriptional responses to short-term exposure to brassinin and then used additional functional approaches. This study supports the hypothesis that indolic phytoalexin primarily targets mitochondrial functions in fungal cells. Indeed, we notably observed that phytoalexin treatment of A. brassicicola disrupted the mitochondrial membrane potential and resulted in a significant and rapid decrease in the oxygen consumption rates. Secondary effects, such as Reactive oxygen species production, changes in lipid and endoplasmic reticulum homeostasis were then found to be induced. Consequently, the fungus has to adapt its metabolism to protect itself against the toxic effects of these molecules, especially via the activation of high osmolarity glycerol and cell wall integrity signaling pathways and by induction of the unfolded protein response. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 N’Guyen, Raulo, Porquier, Iacomi, Pelletier, Renou, Bataillé-Simoneau, Campion, Hamon, Kwasiborski, Colou, Benamar, Hudhomme, Macherel, Simoneau and Guillemette.)
Databáze:	MEDLINE
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