Controlling water transport between micelles and aqueous microdroplets during sample enrichment.

Autor: Fukuyama M; Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1 Katahira, Aoba-ku Sendai, 980-8577, Japan; PREST, Japan Science and Technology, 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012, Japan. Electronic address: maofukuyama@tohoku.ac.jp., Zhou L; Department of Chemistry, Tokyo Institute of Technology, 2-12-1-W4-20, Ookayama, Meguro-ku, Tokyo, 152-8551, Japan., Okada T; Department of Chemistry, Tokyo Institute of Technology, 2-12-1-W4-20, Ookayama, Meguro-ku, Tokyo, 152-8551, Japan., Simonova KV; Chemistry Department, Lomonosov Moscow State University, Moscow, 119991, Russia., Proskurnin M; Chemistry Department, Lomonosov Moscow State University, Moscow, 119991, Russia., Hibara A; Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1 Katahira, Aoba-ku Sendai, 980-8577, Japan. Electronic address: hibara@tohoku.ac.jp.
Jazyk: angličtina
Zdroj: Analytica chimica acta [Anal Chim Acta] 2021 Mar 08; Vol. 1149, pp. 338212. Date of Electronic Publication: 2021 Jan 11.
DOI: 10.1016/j.aca.2021.338212
Abstrakt: Droplet microfluidics technologies have advanced rapidly, but enrichment in droplets has still been difficult. To deterministically control the droplet enrichment, the water transport from an aqueous microdroplet in organic continuous phase containing span 80 micelles was investigated. Organic phase containing Span-80-micelles contacted a NaCl aqueous solution to control hydration degree of the micelles, prior to being used in the microfluidic device. Then, the organic phase was continuously applied to the microdroplets trappled in microwells. Here, water was transported from the microdroplet to the organic phase micelles. This spontaneous emulsification process induced the droplet shrinkage and stopped when the microdroplet reached a certain diameter. The micelle hydration degree correlated well with the final water activity of droplets. The enrichment factor can be determined by the initial microdroplet salt concentration and by the micelle hydration degree. As a proof-of-concept experiment, enrichment of fluorescent nanoparticles and dye was demonstrated, and fluorescent resonance energy transfer was observed as expected. Another demonstration of bound-free separation was performed utilizing the avidin-biotin system. This technique has the potential to be a powerful pretreatment method for bioassays in droplet microfluidics.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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