Microbiota-derived short-chain fatty acids do not interfere with SARS-CoV-2 infection of human colonic samples.

Autor: Pascoal LB; Laboratory of Immunoinflammation, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas (UNICAMP) , Campinas, Brazil.; Inflammatory Bowel Disease Research Laboratory, Colorectal Surgery Unit, Department of Surgery, School of Medical Sciences, University of Campinas (UNICAMP) , Campinas, Brazil., Rodrigues PB; Laboratory of Immunoinflammation, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas (UNICAMP) , Campinas, Brazil., Genaro LM; Inflammatory Bowel Disease Research Laboratory, Colorectal Surgery Unit, Department of Surgery, School of Medical Sciences, University of Campinas (UNICAMP) , Campinas, Brazil., Gomes ABDSP; Laboratory of Immunoinflammation, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas (UNICAMP) , Campinas, Brazil., Toledo-Teixeira DA; Laboratory of Emerging Viruses, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas (UNICAMP) , Campinas, Brazil., Parise PL; Laboratory of Emerging Viruses, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas (UNICAMP) , Campinas, Brazil., Bispo-Dos-Santos K; Laboratory of Emerging Viruses, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas (UNICAMP) , Campinas, Brazil., Simeoni CL; Laboratory of Emerging Viruses, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas (UNICAMP) , Campinas, Brazil., Guimarães PV; Inflammatory Bowel Disease Research Laboratory, Colorectal Surgery Unit, Department of Surgery, School of Medical Sciences, University of Campinas (UNICAMP) , Campinas, Brazil., Buscaratti LI; Brazilian Laboratory on Silencing Technologies (Blast), Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP) , Campinas, Brazil., Elston JGA; Brazilian Laboratory on Silencing Technologies (Blast), Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP) , Campinas, Brazil., Marques-Souza H; Brazilian Laboratory on Silencing Technologies (Blast), Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP) , Campinas, Brazil., Martins-de-Souza D; Experimental Medicine Research Cluster (EMRC), University of Campinas (UNICAMP) , Campinas, Brazil.; Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP) , Campinas, Brazil., Ayrizono MLS; Inflammatory Bowel Disease Research Laboratory, Colorectal Surgery Unit, Department of Surgery, School of Medical Sciences, University of Campinas (UNICAMP) , Campinas, Brazil., Velloso LA; Laboratory of Cell Signaling, School of Medical Sciences, University of Campinas (UNICAMP) , Campinas, Brazil.; Obesity and Comorbidities Research Center (OCRC), University of Campinas (UNICAMP) , Campinas, Brazil., Proenca-Modena JL; Laboratory of Emerging Viruses, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas (UNICAMP) , Campinas, Brazil.; Experimental Medicine Research Cluster (EMRC), University of Campinas (UNICAMP) , Campinas, Brazil., Moraes-Vieira PMM; Experimental Medicine Research Cluster (EMRC), University of Campinas (UNICAMP) , Campinas, Brazil.; Laboratory of Immunometabolism, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas (UNICAMP) , Campinas, Brazil., Mori MAS; Experimental Medicine Research Cluster (EMRC), University of Campinas (UNICAMP) , Campinas, Brazil.; Laboratory of Aging Biology, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP) , Campinas, Brazil., Farias AS; Experimental Medicine Research Cluster (EMRC), University of Campinas (UNICAMP) , Campinas, Brazil.; Autoimmune Research Laboratory, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas (UNICAMP) , Campinas, Brazil., Vinolo MAR; Laboratory of Immunoinflammation, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas (UNICAMP) , Campinas, Brazil.; Experimental Medicine Research Cluster (EMRC), University of Campinas (UNICAMP) , Campinas, Brazil., Leal RF; Inflammatory Bowel Disease Research Laboratory, Colorectal Surgery Unit, Department of Surgery, School of Medical Sciences, University of Campinas (UNICAMP) , Campinas, Brazil.; Obesity and Comorbidities Research Center (OCRC), University of Campinas (UNICAMP) , Campinas, Brazil.
Jazyk: angličtina
Zdroj: Gut microbes [Gut Microbes] 2021 Jan-Dec; Vol. 13 (1), pp. 1-9.
DOI: 10.1080/19490976.2021.1874740
Abstrakt: Microbiota-derived molecules called short-chain fatty acids (SCFAs) play a key role in the maintenance of the intestinal barrier and regulation of immune response during infectious conditions. Recent reports indicate that SARS-CoV-2 infection changes microbiota and SCFAs production. However, the relevance of this effect is unknown. In this study, we used human intestinal biopsies and intestinal epithelial cells to investigate the impact of SCFAs in the infection by SARS-CoV-2. SCFAs did not change the entry or replication of SARS-CoV-2 in intestinal cells. These metabolites had no effect on intestinal cells' permeability and presented only minor effects on the production of anti-viral and inflammatory mediators. Together our findings indicate that the changes in microbiota composition of patients with COVID-19 and, particularly, of SCFAs do not interfere with the SARS-CoV-2 infection in the intestine.
Databáze: MEDLINE