The number and periodicity of seizures induce cardiac remodeling and changes in micro-RNA expression in rats submitted to electric amygdala kindling model of epilepsy.

Autor: Pansani AP; Department of Physiological Sciences, Institute of Biological Sciences, Federal University of Goiás, Goiânia, Brazil. Electronic address: alinepansani@ufg.br., Ghazale PP; Department of Neurology and Neurosurgery, Federal University of São Paulo, São Paulo, Brazil., Dos Santos EG; Department of Physiological Sciences, Institute of Biological Sciences, Federal University of Goiás, Goiânia, Brazil., Dos Santos Borges K; Department of Physiological Sciences, Institute of Biological Sciences, Federal University of Goiás, Goiânia, Brazil., Gomes KP; Department of Physiological Sciences, Institute of Biological Sciences, Federal University of Goiás, Goiânia, Brazil., Lacerda IS; Department of Physiological Sciences, Institute of Biological Sciences, Federal University of Goiás, Goiânia, Brazil., Castro CH; Department of Physiological Sciences, Institute of Biological Sciences, Federal University of Goiás, Goiânia, Brazil., Mendes EP; Department of Physiological Sciences, Institute of Biological Sciences, Federal University of Goiás, Goiânia, Brazil., Dos Santos FCA; Department of Histology, Institute of Biological Sciences, University of Goiás, Goiânia, Brazil., Biancardi MF; Department of Histology, Institute of Biological Sciences, University of Goiás, Goiânia, Brazil., Nejm MB; Department of Neurology and Neurosurgery, Federal University of São Paulo, São Paulo, Brazil., Dogini DB; Department of Medical Genetics and Genomic Medicine, School of Medical Sciences, State University of Campinas (UNICAMP), Campinas, SP, Brazil., Rabelo LA; Department of Physiology, Institute of Biological and Health Sciences, Federal University of Alagoas, Maceió, Alagoas, Brazil., Nunes-Souza V; Department of Physiological and Pharmacology Sciences, Institute of Biological Sciences, Federal University of Pernambuco, Recife, Pernambuco, Brazil., Scorza FA; Department of Neurology and Neurosurgery, Federal University of São Paulo, São Paulo, Brazil., Colugnati DB; Department of Physiological Sciences, Institute of Biological Sciences, Federal University of Goiás, Goiânia, Brazil.
Jazyk: angličtina
Zdroj: Epilepsy & behavior : E&B [Epilepsy Behav] 2021 Mar; Vol. 116, pp. 107784. Date of Electronic Publication: 2021 Feb 03.
DOI: 10.1016/j.yebeh.2021.107784
Abstrakt: Generalized tonic-clonic seizures (GTCS) are the main risk factor for sudden unexpected death in epilepsy (SUDEP). Also, among the several mechanisms underlying SUDEP there is the cardiac dysfunction. So, we aimed to evaluate the impact of the number of seizures on heart function and morphology in rats with epilepsy. Rats were randomized into three groups: Sham (without epilepsy), 5 S, and 10 S groups, referred as rats with epilepsy with a total of 5 or 10 GTCS, respectively. Epilepsy was induced by electrical amygdala kindling. The ventricular function was analyzed by the Langendorff technique and challenged by ischemia/reperfusion protocol. Cardiac fibrosis and hypertrophy were analyzed by histology. We also analyzed cardiac metalloproteinases (MMP2 and MMP9), ERK 1/2 and phosphorylated ERK1/2 (P-ERK) by western blot; microRNA-21 and -320 by RT-PCR; and oxidative stress (TBARS, catalase activity and nitrite) by biochemical analysis. Only the 5S group presented decreased values of ventricular function at before ischemia/reperfusion (baseline): intraventricular systolic pressure, developed intraventricular pressure, positive and negative dP/dt. During ischemia/reperfusion protocol, the variation of the ventricular function did not differ among groups. Both 5S and 10S groups had increased cardiomyocyte hypertrophy and fibrosis compared to Sham, but in the 5S group, these alterations were higher than in the 10S group. The 5S group increased in microRNA-21 and decreased in microRNA-320 expression compared to Sham and the 10S group. The 10S group increased in MMP9 and decreased in P-ERK/ERK expression, and increased in nitrite content compared to both Sham and the 5S group. Therefore, seizures impair cardiac function and morphology, probably through microRNA modulation. The continuation of seizures seems to exert a preconditioning-like stimulus that fails to compensate the cardiac tissue alteration.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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