Vasohibin 1 selectively regulates secondary sprouting and lymphangiogenesis in the zebrafish trunk.

Autor:	de Oliveira MB; Integrative Vascular Biology Laboratory, Max-Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Robert-Rössle-Strasse 10, Berlin 13125, Germany.; DZHK (German Center for Cardiovascular Research), Partner site, Potsdamer Str. 58, 10785 Berlin, Germany., Meier K; Integrative Vascular Biology Laboratory, Max-Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Robert-Rössle-Strasse 10, Berlin 13125, Germany.; DZHK (German Center for Cardiovascular Research), Partner site, Potsdamer Str. 58, 10785 Berlin, Germany., Jung S; Integrative Vascular Biology Laboratory, Max-Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Robert-Rössle-Strasse 10, Berlin 13125, Germany.; DZHK (German Center for Cardiovascular Research), Partner site, Potsdamer Str. 58, 10785 Berlin, Germany., Bartels-Klein E; Integrative Vascular Biology Laboratory, Max-Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Robert-Rössle-Strasse 10, Berlin 13125, Germany.; DZHK (German Center for Cardiovascular Research), Partner site, Potsdamer Str. 58, 10785 Berlin, Germany., Coxam B; Integrative Vascular Biology Laboratory, Max-Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Robert-Rössle-Strasse 10, Berlin 13125, Germany.; DZHK (German Center for Cardiovascular Research), Partner site, Potsdamer Str. 58, 10785 Berlin, Germany., Geudens I; Department of Immunology, Genetics and Pathology, Uppsala University, 752 37 Uppsala, Sweden.; Vascular Patterning Laboratory, Center for Cancer Biology, VIB, Leuven B-3000, Belgium., Szymborska A; Integrative Vascular Biology Laboratory, Max-Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Robert-Rössle-Strasse 10, Berlin 13125, Germany.; DZHK (German Center for Cardiovascular Research), Partner site, Potsdamer Str. 58, 10785 Berlin, Germany., Skoczylas R; Department of Immunology, Genetics and Pathology, Uppsala University, 752 37 Uppsala, Sweden., Fechner I; Integrative Vascular Biology Laboratory, Max-Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Robert-Rössle-Strasse 10, Berlin 13125, Germany.; DZHK (German Center for Cardiovascular Research), Partner site, Potsdamer Str. 58, 10785 Berlin, Germany., Koltowska K; Department of Immunology, Genetics and Pathology, Uppsala University, 752 37 Uppsala, Sweden., Gerhardt H; Integrative Vascular Biology Laboratory, Max-Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Robert-Rössle-Strasse 10, Berlin 13125, Germany holger.gerhardt@mdc-berlin.de.; DZHK (German Center for Cardiovascular Research), Partner site, Potsdamer Str. 58, 10785 Berlin, Germany.; Vascular Patterning Laboratory, Center for Cancer Biology, VIB, Leuven B-3000, Belgium.; Vascular Patterning Laboratory, Center for Cancer Biology, Department of Oncology, KU Leuven, Leuven B-3000, Belgium.; Berlin Institute of Health (BIH), Anna-Louisa-Karsch-Straβe 2, 10178 Berlin, Germany.
Jazyk:	angličtina
Zdroj:	Development (Cambridge, England) [Development] 2021 Feb 19; Vol. 148 (4). Date of Electronic Publication: 2021 Feb 19.
DOI:	10.1242/dev.194993
Abstrakt:	Previous studies have shown that Vasohibin 1 (Vash1) is stimulated by VEGFs in endothelial cells and that its overexpression interferes with angiogenesis in vivo Recently, Vash1 was found to mediate tubulin detyrosination, a post-translational modification that is implicated in many cell functions, such as cell division. Here, we used the zebrafish embryo to investigate the cellular and subcellular mechanisms of Vash1 on endothelial microtubules during formation of the trunk vasculature. We show that microtubules within venous-derived secondary sprouts are strongly and selectively detyrosinated in comparison with other endothelial cells, and that this difference is lost upon vash1 knockdown. Vash1 depletion in zebrafish specifically affected secondary sprouting from the posterior cardinal vein, increasing endothelial cell divisions and cell number in the sprouts. We show that altering secondary sprout numbers and structure upon Vash1 depletion leads to defective lymphatic vessel formation and ectopic lymphatic progenitor specification in the zebrafish trunk. Competing Interests: Competing interestsThe authors declare no competing or financial interests. (© 2021. Published by The Company of Biologists Ltd.)
Databáze:	MEDLINE
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