| Autor: |
Yehuda N; Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel., Turkulets Y; Department of Electrical and Computer Engineering, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel., Shalish I; Department of Electrical and Computer Engineering, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel., Kushmaro A; Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel.; The Ilse Katz Center for Meso and Nanoscale Science and Technology, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel., Malis Arad S; Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel. |
| Jazyk: |
angličtina |
| Zdroj: |
ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2021 Feb 17; Vol. 13 (6), pp. 7070-7079. Date of Electronic Publication: 2021 Feb 05. |
| DOI: |
10.1021/acsami.0c17919 |
| Abstrakt: |
The anion-exchange capacity of the cell-wall sulfated polysaccharide of the red microalga Porphyridium sp. can be exploited for the complexation of metal ions (e.g., Cu, Zn, Ag) to produce novel materials with new bioactivities. In this study, we investigated this algal polysaccharide as a platform for the incorporation of copper as Cu 2 O. Chemical and rheological characterization of the Cu 2 O-polysaccharide complex showed that the copper is covalently bound to the polysaccharide and that the complex exhibits higher viscosity and conductivity than the native polysaccharide. Examination of the complex's inhibitory activity against the bacteria Acinetobacter baumannii , Pseudomonas aeruginosa , Escherichia coli , Staphylococcus aureus , and Bacillus subtilis and the fungus Candida albicans revealed a relatively high antimicrobial activity, especially against C. albicans (92% growth inhibition) as compared to the polysaccharide and to Cu 2 O alone. The antibiofilm activity was also found against P. aeruginosa PA14 and C. albicans biofilms. An atomic force microscopy examination of the surface morphology of the complex revealed needle-like structures (spikes), approximately 10 nm thick, protruding from the complex surface to a maximum height of 1000 nm, at a density of about 5000/μm 2 , which were not detected in the native polysaccharide. It seems that the spikes on the surface of the Cu 2 O-polysaccharide complex are responsible for the antimicrobial activities of the complex, that is, for disruption of microbial membrane permeability, leading to cell death. The study thus indicates that the superior qualities of the novel material formed by complexion of Cu 2 O to the polysaccharide should be studied further for various biotechnological applications. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
