SPOCK, an R based package for high-throughput analysis of growth rate, survival, and chronological lifespan in yeast.

Autor:	Small EM; Department of Molecular Genetics and Microbiology, University of New Mexico Health Sciences Center, Albuquerque, NM, 87131, USA., Felker DP; Department of Biochemistry and Molecular Biology, School of Medicine, University of New Mexico Health Sciences Center, Albuquerque, NM, 87131, USA., Heath OC; Department of Biochemistry and Molecular Biology, School of Medicine, University of New Mexico Health Sciences Center, Albuquerque, NM, 87131, USA., Cantergiani RJ; Department of Biochemistry and Molecular Biology, School of Medicine, University of New Mexico Health Sciences Center, Albuquerque, NM, 87131, USA., Osley MA; Department of Molecular Genetics and Microbiology, University of New Mexico Health Sciences Center, Albuquerque, NM, 87131, USA., McCormick MA; Department of Biochemistry and Molecular Biology, School of Medicine, University of New Mexico Health Sciences Center, Albuquerque, NM, 87131, USA.; Autophagy Inflammation and Metabolism Center of Biomedical Research Excellence, USA.
Jazyk:	angličtina
Zdroj:	Translational medicine of aging [Transl Med Aging] 2020; Vol. 4, pp. 141-148. Date of Electronic Publication: 2020 Aug 21.
DOI:	10.1016/j.tma.2020.08.003
Abstrakt:	Plate reader-based methods for high-throughput measurement of growth rate, cellular survival, and chronological lifespan are a compelling addition to the already powerful toolbox of budding yeast Saccharomyces cerevisiae genetics. These methods have overcome many of the limits of traditional yeast biology techniques, but also present a new bottleneck at the point of data-analysis. Herein, we describe SPOCK (Survival Percentage and Outgrowth Collection Kit), an R-based package for the analysis of data created by high-throughput plate reader based methods. This package allows for the determination of chronological lifespan, cellular growth rate, and survival in an efficient, robust, and reproducible fashion. Competing Interests: DECLARATION OF COMPETING INTERESTS The authors do not have any conflicts of interest to declare.
Databáze:	MEDLINE
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