A shape-adjusted ellipse approach corrects for varied axonal dispersion angles and myelination in primate nerve roots.

Autor: Bartmeyer PM; Department of Neurology, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.; School of Electrical and Computer Engineering at University of Campinas, Campinas, SP, Brazil., Biscola NP; Department of Neurology, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.; Department of Neurology, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA., Havton LA; Department of Neurology, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA. leif.havton@mssm.edu.; Departments of Neurology and Neurobiology, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA. leif.havton@mssm.edu.; Departments of Neurology and Neuroscience, Icahn School of Medicine at Mount Sinai, New York, NY, USA. leif.havton@mssm.edu.; Neurology Service and RR&D National Center for the Medical Consequences of Spinal Cord Injury, James J. Peters Veterans Administration Medical Center, Bronx, NY, USA. leif.havton@mssm.edu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2021 Feb 04; Vol. 11 (1), pp. 3150. Date of Electronic Publication: 2021 Feb 04.
DOI: 10.1038/s41598-021-82575-9
Abstrakt: Segmentation of axons in light and electron micrographs allows for quantitative high-resolution analysis of nervous tissues, but varied axonal dispersion angles result in over-estimates of fiber sizes. To overcome this technical challenge, we developed a novel shape-adjusted ellipse (SAE) determination of axonal size and myelination as an all-inclusive and non-biased tool to correct for oblique nerve fiber presentations. Our new resource was validated by light and electron microscopy against traditional methods of determining nerve fiber size and myelination in rhesus macaques as a model system. We performed detailed segmental mapping and characterized the morphological signatures of autonomic and motor fibers in primate lumbosacral ventral roots (VRs). An en bloc inter-subject variability for the preganglionic parasympathetic fibers within the L7-S2 VRs was determined. The SAE approach allows for morphological ground truth data collection and assignment of individual axons to functional phenotypes with direct implications for fiber mapping and neuromodulation studies.
Databáze: MEDLINE
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