A ketogenic diet differentially affects neuron and astrocyte transcription.

Autor: Koppel SJ; University of Kansas Alzheimer's Disease Center, Kansas City, KS, USA.; Departments of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS, USA., Pei D; Departments of Biostatistics and Data Science, University of Kansas Medical Center, Kansas City, KS, USA., Wilkins HM; University of Kansas Alzheimer's Disease Center, Kansas City, KS, USA.; Departments of Neurology, University of Kansas Medical Center, Kansas City, KS, USA., Weidling IW; University of Kansas Alzheimer's Disease Center, Kansas City, KS, USA.; Departments of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS, USA., Wang X; University of Kansas Alzheimer's Disease Center, Kansas City, KS, USA.; Departments of Neurology, University of Kansas Medical Center, Kansas City, KS, USA., Menta BW; University of Kansas Alzheimer's Disease Center, Kansas City, KS, USA.; Departments of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS, USA., Perez-Ortiz J; University of Kansas Alzheimer's Disease Center, Kansas City, KS, USA.; Departments of Neurology, University of Kansas Medical Center, Kansas City, KS, USA., Kalani A; University of Kansas Alzheimer's Disease Center, Kansas City, KS, USA.; Departments of Neurology, University of Kansas Medical Center, Kansas City, KS, USA., Manley S; University of Kansas Alzheimer's Disease Center, Kansas City, KS, USA.; Departments of Neurology, University of Kansas Medical Center, Kansas City, KS, USA., Novikova L; University of Kansas Alzheimer's Disease Center, Kansas City, KS, USA.; Departments of Neurology, University of Kansas Medical Center, Kansas City, KS, USA., Koestler DC; Departments of Biostatistics and Data Science, University of Kansas Medical Center, Kansas City, KS, USA., Swerdlow RH; University of Kansas Alzheimer's Disease Center, Kansas City, KS, USA.; Departments of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS, USA.; Departments of Neurology, University of Kansas Medical Center, Kansas City, KS, USA.; Departments of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS, USA.
Jazyk: angličtina
Zdroj: Journal of neurochemistry [J Neurochem] 2021 Jun; Vol. 157 (6), pp. 1930-1945. Date of Electronic Publication: 2021 Feb 23.
DOI: 10.1111/jnc.15313
Abstrakt: Ketogenic diets (KDs) alter brain metabolism. Multiple mechanisms may account for their effects, and different brain regions may variably respond. Here, we considered how a KD affects brain neuron and astrocyte transcription. We placed male C57Bl6/N mice on either a 3-month KD or chow diet, generated enriched neuron and astrocyte fractions, and used RNA-Seq to assess transcription. Neurons from KD-treated mice generally showed transcriptional pathway activation while their astrocytes showed a mix of transcriptional pathway suppression and activation. The KD especially affected pathways implicated in mitochondrial and endoplasmic reticulum function, insulin signaling, and inflammation. An unbiased analysis of KD-associated expression changes strongly implicated transcriptional pathways altered in AD, which prompted us to explore in more detail the potential molecular relevance of a KD to AD. Our results indicate a KD differently affects neurons and astrocytes, and provide unbiased evidence that KD-induced brain effects are potentially relevant to neurodegenerative diseases such as AD.
(© 2021 International Society for Neurochemistry.)
Databáze: MEDLINE
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