Data about performances of whole and monovalent half-fragments antibodies in immunosorbent assays.

Autor:	Susini V; Department of Translational Research and of New Surgical and Medical Technologies, University of Pisa, via Savi 10, Pisa, Italy., Caponi L; Department of Translational Research and of New Surgical and Medical Technologies, University of Pisa, via Savi 10, Pisa, Italy., Rossi VL; bioMérieux Italia Spa, Via di Campigliano, 58, 50012 Bagno a Ripoli, Florence, Italy., Sanesi A; bioMérieux Italia Spa, Via di Campigliano, 58, 50012 Bagno a Ripoli, Florence, Italy., Romiti N; Department of Translational Research and of New Surgical and Medical Technologies, University of Pisa, via Savi 10, Pisa, Italy., Paolicchi A; Department of Translational Research and of New Surgical and Medical Technologies, University of Pisa, via Savi 10, Pisa, Italy., Franzini M; Department of Translational Research and of New Surgical and Medical Technologies, University of Pisa, via Savi 10, Pisa, Italy.
Jazyk:	angličtina
Zdroj:	Data in brief [Data Brief] 2021 Jan 20; Vol. 35, pp. 106778. Date of Electronic Publication: 2021 Jan 20 (Print Publication: 2021).
DOI:	10.1016/j.dib.2021.106778
Abstrakt:	The data here presented are related to the research article entitled "Sensitivity and reproducibility enhancement in enzyme immunosorbent assays based on half fragment antibodies" [1] aimed to compare the performance in ELISA of whole antibodies and their corresponding monovalent half-fragments obtained by reduction. Half-fragment antibodies represent an interesting method to orient antibodies in high-sensitive immunoassays taking advantage of the free sulfhydryl groups of the hinge region [2], [3], [4] that allow their oriented binding on maleimide functionalized microplates. Data here presented describe the contribution of both chemical reduction and orientation on the antigen binding capacity of whole and half-fragments antibodies. For this purpose, monoclonal anti-horseradish peroxidase (anti-HRP) or monoclonal anti-fPSA antibodies, and their respective half-fragments, were coated on polystyrene or maleimide functionalized microplates. The antigen binding capability was analyzed by in-house enzyme linked immunosorbent assays. These data would be used for further studies on the development of oriented immunoassays based on half fragment antibodies. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships which have, or could be perceived to have, influenced the work reported in this article. (© 2021 The Author(s).)
Databáze:	MEDLINE
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