The Isolation and Manufacture of GMP-Grade Bone Marrow Stromal Cells from Bone Specimens.

Autor:	Dias RB; Research Division, National Institute of Traumatology and Orthopedics, Rio de Janeiro, Brazil.; Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Bonfim DC; Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil. bonfimdc@icb.ufrj.br.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2286, pp. 95-105.
DOI:	10.1007/7651_2020_319
Abstrakt:	Bone marrow stromal cells (BMSCs, also known as bone marrow mesenchymal stem cells) are a plastic-adherent heterogeneous cell population that contain inherent skeletal progenitors and a subset of multipotential skeletal stem cells (SSCs). Application of BMSCs in therapeutic protocols implies its isolation and expansion under good manufacturing practices (GMP). Here we describe the procedures we have found to successfully generate practical BMSCs numbers, with preserved biological potency.
Databáze:	MEDLINE
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