Dendritic cell actin dynamics control contact duration and priming efficiency at the immunological synapse.

Autor:	Leithner A; Institute of Science and Technology Austria, Klosterneuburg, Austria.; Kennedy Institute of Rheumatology, University of Oxford, Oxford, UK., Altenburger LM; Department of Oncology, Microbiology and Immunology, University of Fribourg, Fribourg, Switzerland., Hauschild R; Institute of Science and Technology Austria, Klosterneuburg, Austria., Assen FP; Institute of Science and Technology Austria, Klosterneuburg, Austria., Rottner K; Zoological Institute, Technical University Braunschweig, Braunschweig, Germany.; Department of Cell Biology, Helmholtz Centre for Infection Research, Braunschweig, Germany., Stradal TEB; Department of Cell Biology, Helmholtz Centre for Infection Research, Braunschweig, Germany., Diz-Muñoz A; Cell Biology and Biophysics Units, European Molecular Biology Laboratory, Heidelberg, Germany., Stein JV; Department of Oncology, Microbiology and Immunology, University of Fribourg, Fribourg, Switzerland., Sixt M; Institute of Science and Technology Austria, Klosterneuburg, Austria.
Jazyk:	angličtina
Zdroj:	The Journal of cell biology [J Cell Biol] 2021 Apr 05; Vol. 220 (4).
DOI:	10.1083/jcb.202006081
Abstrakt:	Dendritic cells (DCs) are crucial for the priming of naive T cells and the initiation of adaptive immunity. Priming is initiated at a heterologous cell-cell contact, the immunological synapse (IS). While it is established that F-actin dynamics regulates signaling at the T cell side of the contact, little is known about the cytoskeletal contribution on the DC side. Here, we show that the DC actin cytoskeleton is decisive for the formation of a multifocal synaptic structure, which correlates with T cell priming efficiency. DC actin at the IS appears in transient foci that are dynamized by the WAVE regulatory complex (WRC). The absence of the WRC in DCs leads to stabilized contacts with T cells, caused by an increase in ICAM1-integrin-mediated cell-cell adhesion. This results in lower numbers of activated and proliferating T cells, demonstrating an important role for DC actin in the regulation of immune synapse functionality. (© 2021 Leithner et al.)
Databáze:	MEDLINE
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