[Decreased DNA hydroxymethylation and increased DNA demethylation are associated with high antioxidant response in systemic lupus erythematosus patients].

Autor:	Fragío Gil JJ; Rheumatology Department, Hospital Universitari i Politècnic La Fe, Valencia, Spain; Escuela de Doctorado, Universidad Católica de Valencia San Vicente Mártir, Valencia, Spain., Grau García E; Rheumatology Department, Hospital Universitari i Politècnic La Fe, Valencia, Spain. Electronic address: grau_elegar@gva.es., Fernández Matilla M; Rheumatology Section, Hospital Arnau de Vilanova, Valencia, Spain., Ortiz Sanjuan FM; Rheumatology Department, Hospital Universitari i Politècnic La Fe, Valencia, Spain., Rodrigo Nicolás R; Molecular, Cellular and Genomic Biomedicine Group, IIS La Fe, Valencia, Spain; CIBERER, Spain., Román Ivorra JA; Rheumatology Department, Hospital Universitari i Politècnic La Fe, Valencia, Spain; Escuela de Doctorado, Universidad Católica de Valencia San Vicente Mártir, Valencia, Spain.
Jazyk:	English; Spanish; Castilian
Zdroj:	Medicina clinica [Med Clin (Barc)] 2021 Dec 24; Vol. 157 (12), pp. 575-579. Date of Electronic Publication: 2021 Jan 30.
DOI:	10.1016/j.medcli.2020.10.023
Abstrakt:	Objectives: DNA hydroxymethylation may be induced by oxidative stress in lupus patients, so we investigated the association between DNA hydroxymethylation and demethylation with the antioxidant response. Methods: A case-control study was performed including lupus patients and matched healthy controls. Serum concentration of glutathione (GSH), glutathione disulphide (GSSG), superoxide dismutase (SOD) and total antioxidant capacity (TAC), 5-mC and 5-hmC were determined. Results: One hundred and forty-two patients and 34 controls were included. 5-hmC levels were lower in SLE patients than in controls. GSH and GSSG values were lower in patients, while SOD levels were higher in patients. TAC did not show significant differences, but higher demethylation and lower hydroxydemethylation were associated to increased TAC values. Lower demethylation was associated with cytopenia and lower hydroxymethylation with longer course of the disease. Lower levels of GSH and GSSG and higher SOD values were associated with accumulated damage assessed by SLICC-ACR. Conclusions: Lower hydroxymethylation in patients than in controls was observed. Moreover, higher demethylation and lower hydroxymethylation leads to high TAC levels. DNA hydroxymethylation seems to be related to longer course of the disease. (Copyright © 2020 Elsevier España, S.L.U. All rights reserved.)
Databáze:	MEDLINE
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