Biochemical analysis of leptospiral LPS explained the difference between pathogenic and non-pathogenic serogroups.

Autor: Vanithamani S; Medical Microbiology Laboratory, Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620024, India., Akino Mercy CS; Medical Microbiology Laboratory, Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620024, India., Kanagavel M; Medical Microbiology Laboratory, Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620024, India., Sumaiya K; Medical Microbiology Laboratory, Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620024, India., Bothammal P; Medical Microbiology Laboratory, Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620024, India., Saranya P; Medical Microbiology Laboratory, Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620024, India., Prasad M; Medical Microbiology Laboratory, Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620024, India., Ponmurugan K; Department of Botany & Microbiology, College of Science, King Saud University, P.O.Box 2455, Riyadh, 11451, Saudi Arabia., Muralitharan G; Medical Microbiology Laboratory, Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620024, India., Al-Dhabi NA; Department of Botany & Microbiology, College of Science, King Saud University, P.O.Box 2455, Riyadh, 11451, Saudi Arabia., Verma A; Lincoln Memorial University, College of Veterinary Medicine, Harrogate, TN, 37752, USA., Vijayachari P; WHO Collaborating Centre for Diagnosis, Reference, Research and Training in Leptospirosis, Regional Medical Research Centre (ICMR), Port Blair, 744103, India., Natarajaseenivasan K; Medical Microbiology Laboratory, Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620024, India; Department of Neuroscience, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, 19140, USA. Electronic address: natarajaseenivasan@gmail.com.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2021 Mar; Vol. 152, pp. 104738. Date of Electronic Publication: 2021 Jan 30.
DOI: 10.1016/j.micpath.2021.104738
Abstrakt: Lipopolysaccharide (LPS) is the major surface antigen of Leptospira. In this study, the genes involved in the LPS biosynthesis were analyzed and compared by bioinformatics tools. Also, the chemical composition analysis of leptospiral lipopolysaccharides (LPS) extracted from 5 pathogenic serovars like Autumnalis, Australis, Ballum, Grippotyphosa, Pomona, and the nonpathogenic serovar Andamana was performed. Methods used were Limulus amebocyte lysate assay (LAL), gas chromatography-mass spectrometry (GC-MS), fourier transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance spectroscopy (NMR). LAL assay showed a significantly higher level of endotoxicity among pathogenic serovars (~0.490 EU/mL) than that of nonpathogenic Andamana (~0.102 EU/mL). FAMES analysis showed the presence of palmitic acid (C16:0), hydroxy lauric acid (3-OH-C12:0), and oleic acid (C18:0). Palmitoleic acid (C16: 1), and 3- hydroxy palmitate (3-OH-C16:0) was detected only in pathogenic serovars. In contrast myristoleic acid (C14:1) and stearic acid (C18:0) were present in Andamana. FTIR analysis revealed C-O-C stretch of esters, 3°ROH functional groups and carbohydrate vibration range were similar among pathogenic serovars. The NMR analysis reveals similarity for 6 deoxy sugars and methyl groups of Autumnalis, Australis, and Ballum. Further, the presence of palmitoleic acid and 3-hydroxy palmitate may be the significant pathogen-associated predisposing factor. This mediates high osmolarity glycerol (HOG) mediated stress response in leptospiral LPS mediated pathogenesis.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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