| Autor: |
Liang J; School of Agriculture and Biology, Shanghai Jiao Tong University/Key Laboratory of Urban Agriculture by Ministry of Agriculture of China, Shanghai 200240, PR China., Wang S; School of Agriculture and Biology, Shanghai Jiao Tong University/Key Laboratory of Urban Agriculture by Ministry of Agriculture of China, Shanghai 200240, PR China., Yiming A; School of Agriculture and Biology, Shanghai Jiao Tong University/Key Laboratory of Urban Agriculture by Ministry of Agriculture of China, Shanghai 200240, PR China., Fu L; School of Agriculture and Biology, Shanghai Jiao Tong University/Key Laboratory of Urban Agriculture by Ministry of Agriculture of China, Shanghai 200240, PR China., Ahmad I; Department of Environmental Sciences, COMSATS University Islamabad, Vehari-Campus, Vehari-61100, Pakistan.; School of Agriculture and Biology, Shanghai Jiao Tong University/Key Laboratory of Urban Agriculture by Ministry of Agriculture of China, Shanghai 200240, PR China., Chen G; School of Agriculture and Biology, Shanghai Jiao Tong University/Key Laboratory of Urban Agriculture by Ministry of Agriculture of China, Shanghai 200240, PR China., Zhu B; School of Agriculture and Biology, Shanghai Jiao Tong University/Key Laboratory of Urban Agriculture by Ministry of Agriculture of China, Shanghai 200240, PR China. |
| Abstrakt: |
Strain L22-9 T , a Gram-stain-negative and rod-shaped bacterium, motile by one polar flagellum, was isolated from cornfield soil in Bijie, Guizhou Province, PR China. Based on 16S rRNA gene sequences, it was identified as a Pseudomonas species. Multilocus sequence analysis of concatenated 16S rRNA, gyrB , rpoB and rpoD gene sequences showed that strain L22-9 T formed a clearly separated branch, located in a cluster together with Pseudomonas brassicacearum LMG 21623 T , Pseudomonas kilonensis DSM 13647 T and Pseudomonas thivervalensis DSM 13194 T . Whole-genome comparisons based on average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) confirmed that strain L22-9 T should be classified as a novel species. It was most closely related to P. kilonensis DSM 13647 T with ANI and dDDH values of 91.87 and 46.3 %, respectively. Phenotypic features that can distinguish strain L22-9 T from P. kilonensis DSM 13647 T are the assimilation ability of N -acetyl-d-glucosamine, poor activity of arginine dihydrolase and failure to ferment ribose and d-fucose. The predominant cellular fatty acids of strain L22-9 T are C 16 : 0 , summed feature 3 (C 16 : 1 ω6 c and/or C 16 : 1 ω7 c ) and summed feature 8 (C 18 : 1 ω7 c and/or C 18 : 1 ω6 c ). The respiratory quinones consist of Q-9 and Q-8. The polar lipids are diphosphatidylglycerol, phosphatidylethanolamine, two unidentified phosphoglycolipids, two unidentified aminophospholipids and an unidentified glycolipid. Based on the evidence, we conclude that strain L22-9 T represents a novel species, for which the name Pseudomonas bijieensis sp. nov. is proposed. The type strain is L22-9 T (=CGMCC 1.18528 T =LMG 31948 T ), with a DNA G+C content of 60.85 mol%. |
