| Autor: |
Kramina TE; Department of Higher Plants, Biological Faculty, Lomonosov Moscow State University, GSP-1, Leninskie Gory, 119234 Moscow, Russia., Lysova MV; LLC 'Amplitech', 1-ya Kuryanovskaya Str., 34-8, 109235 Moscow, Russia., Samigullin TH; A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, GSP-1, Leninskie Gory, 119991 Moscow, Russia., Schanzer IA; Tsitsin Main Botanical Garden of Russian Academy of Sciences, Botanicheskaya Str., 4, 127276 Moscow, Russia., Özbek MU; Department of Biology, Faculty of Science, Gazi University, 06500 Teknikokullar, Ankara, Turkey., Sokoloff DD; Department of Higher Plants, Biological Faculty, Lomonosov Moscow State University, GSP-1, Leninskie Gory, 119234 Moscow, Russia. |
| Abstrakt: |
The Mediterranean region is a center of species and genetic diversity of many plant groups, which served as a source of recolonization of temperate regions of Eurasia in Holocene. We investigate the evolutionary history of species currently classified in Lotus sect. Bonjeanea in the context of the evolution of the genus Lotus as a whole, using phylogenetic, phylogeographic and dating analyses. Of three species of the section, L. rectus and L. hirsutus have wide Mediterranean distribution while L. strictus has a disjunctive range in Bulgaria, Turkey, Armenia, Eastern Kazakhstan, and adjacent parts of Russia and China. We used entire nuclear ribosomal ITS1-5.8S-ITS2 region (nrITS) and a plastid dataset ( rps 16 and trn L-F) to reconstruct phylogenetic relationships within Lotus with an extended representation of Bonjeanea group. We analyzed the phylogeographic patterns within each species based on the plastid dataset. For divergence time estimation, the nrITS dataset was analyzed. Our results confirmed the non-monophyletic nature of the section Bonjeanea . They indicate that Lotus is likely to have diverged about 15.87 (9.99-19.81) million years ago (Ma), which is much older than an earlier estimate of ca. 5.54 Ma. Estimated divergence ages within L. strictus , L. rectus , and L. hisrutus (6.1, 4.94, and 4.16 Ma, respectively) well predate the onset of the current type of Mediterranean climate. Our data suggest that relatively ancient geological events and/or climatic changes apparently played roles in early diversification of Lotus and its major clades, as well as in formation of phylogeographic patterns, in at least some species. |
