Nicotinamide Riboside Enhances In Vitro Beta-adrenergic Brown Adipose Tissue Activity in Humans.

Autor:	Nascimento EBM; NUTRIM School of Nutrition and Translational Research in Metabolism; Department of Nutrition and Movement Sciences; Maastricht University Medical Center, Maastricht, MD, The Netherlands., Moonen MPB; NUTRIM School of Nutrition and Translational Research in Metabolism; Department of Nutrition and Movement Sciences; Maastricht University Medical Center, Maastricht, MD, The Netherlands., Remie CME; NUTRIM School of Nutrition and Translational Research in Metabolism; Department of Nutrition and Movement Sciences; Maastricht University Medical Center, Maastricht, MD, The Netherlands., Gariani K; Laboratory of Integrative and Systems Physiology, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.; Division of Endocrinology, Diabetes, Nutrition and Therapeutic Patient Education, Geneva University Hospitals, Geneva, Switzerland., Jörgensen JA; NUTRIM School of Nutrition and Translational Research in Metabolism; Department of Nutrition and Movement Sciences; Maastricht University Medical Center, Maastricht, MD, The Netherlands., Schaart G; NUTRIM School of Nutrition and Translational Research in Metabolism; Department of Nutrition and Movement Sciences; Maastricht University Medical Center, Maastricht, MD, The Netherlands., Hoeks J; NUTRIM School of Nutrition and Translational Research in Metabolism; Department of Nutrition and Movement Sciences; Maastricht University Medical Center, Maastricht, MD, The Netherlands., Auwerx J; Laboratory of Integrative and Systems Physiology, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland., van Marken Lichtenbelt WD; NUTRIM School of Nutrition and Translational Research in Metabolism; Department of Nutrition and Movement Sciences; Maastricht University Medical Center, Maastricht, MD, The Netherlands., Schrauwen P; NUTRIM School of Nutrition and Translational Research in Metabolism; Department of Nutrition and Movement Sciences; Maastricht University Medical Center, Maastricht, MD, The Netherlands.
Jazyk:	angličtina
Zdroj:	The Journal of clinical endocrinology and metabolism [J Clin Endocrinol Metab] 2021 Apr 23; Vol. 106 (5), pp. 1437-1447.
DOI:	10.1210/clinem/dgaa960
Abstrakt:	Context: Elevating nicotinamide adenine dinucleotide (NAD+) levels systemically improves metabolic health, which can be accomplished via nicotinamide riboside (NR). Previously, it was demonstrated that NR supplementation in high-fat-diet (HFD)-fed mice decreased weight gain, normalized glucose metabolism, and enhanced cold tolerance. Objective: Because brown adipose tissue (BAT) is a major source of thermogenesis, we hypothesize that NR stimulates BAT in mice and humans. Design and Intervention: HFD-fed C56BL/6J mice were supplemented with 400 mg/kg/day NR for 4 weeks and subsequently exposed to cold. In vitro primary adipocytes derived from human BAT biopsies were pretreated with 50 µM or 500 µM NR before measuring mitochondrial uncoupling. Human volunteers (45-65 years; body mass index, 27-35 kg/m2) were supplemented with 1000 mg/day NR for 6 weeks to determine whether BAT activity increased, as measured by [18F]FDG uptake via positron emission tomography-computed tomography (randomized, double blinded, placebo-controlled, crossover study with NR supplementation). Results: NR supplementation in HFD-fed mice decreased adipocyte cell size in BAT. Cold exposure further decreased adipocyte cell size on top of that achieved by NR alone independent of ex vivo lipolysis. In adipocytes derived from human BAT, NR enhanced in vitro norepinephrine-stimulated mitochondrial uncoupling. However, NR supplementation in human volunteers did not alter BAT activity or cold-induced thermogenesis. Conclusions: NR stimulates in vitro human BAT but not in vivo BAT in humans. Our research demonstrates the need for further translational research to better understand the differences in NAD+ metabolism in mouse and human. (© The Author(s) 2021. Published by Oxford University Press on behalf of the Endocrine Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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