In vitro ameliorative effects of ellagic acid on vitality, motility and DNA quality in human spermatozoa.

Autor: Iovine C; Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania 'Luigi Vanvitelli', Caserta, Italy., Mottola F; Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania 'Luigi Vanvitelli', Caserta, Italy., Santonastaso M; Department of Woman, Child and General and Special Surgery, University of Campania 'Luigi Vanvitelli', Napoli, Italy., Finelli R; American Center of Reproductive Medicine, Andrology Center, Cleveland Clinic, Cleveland, Ohio, USA., Agarwal A; American Center of Reproductive Medicine, Andrology Center, Cleveland Clinic, Cleveland, Ohio, USA., Rocco L; Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania 'Luigi Vanvitelli', Caserta, Italy.
Jazyk: angličtina
Zdroj: Molecular reproduction and development [Mol Reprod Dev] 2021 Feb; Vol. 88 (2), pp. 167-174. Date of Electronic Publication: 2021 Jan 31.
DOI: 10.1002/mrd.23455
Abstrakt: Oxidative stress (OS) plays a significant role in the etiology of male infertility, resulting in the impairment of male reproduction. This condition, characterized by an imbalance in the levels of oxidizing and antioxidant species in the seminal fluid, has a harmful impact on sperm functions and DNA integrity. The present study aimed to evaluate the anti-genotoxic action of ellagic acid, a polyphenolic molecule of natural origin having a powerful antigenotoxic, anti-inflammatory and antiproliferative role. An OS condition was induced in vitro by incubating normozoospermic human semen samples in benzene for 45, 60 and 90 min. DNA integrity was evaluated by terminal deoxynucleotidyl transferase dUTP nick end labeling assay, RAPD-PCR was performed to calculate the genome template stability, while the percentage of intracellular reactive oxygen species (ROS) was assessed by the 2', 7'-dichlorofluorescein assay. Our results showed that ellagic acid has a consistent protective effect on DNA integrity, as well as on sperm vitality and motility, by counteracting generation of intracellular ROS. The results of this study suggest ellagic acid as a suitable molecule to protect sperm DNA from oxidative stress, with a potentially significant translational impact on the management of the male infertility.
(© 2021 Wiley Periodicals LLC.)
Databáze: MEDLINE
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