Obtaining hemicellulosic hydrolysate from sugarcane bagasse for microbial oil production by Lipomyces starkeyi.

Autor: da Cunha Abreu Xavier M; Department of Bioprocess Engineering and Biotechnology, Federal University of Tocantins (UFT), Badejos Street 69-72, Jardim Cervilha, Gurupi, TO, 77404-970, Brazil. michellecax@mail.uft.edu.br., Teixeira Franco T; Department of Process Engineering (DEPro), School of Chemical Engineering, State University of Campinas (UNICAMP), Albert Einstein Avenue, 500, Zeferino Vaz University City, Campinas, SP, 13083-852, Brazil.
Jazyk: angličtina
Zdroj: Biotechnology letters [Biotechnol Lett] 2021 May; Vol. 43 (5), pp. 967-979. Date of Electronic Publication: 2021 Jan 31.
DOI: 10.1007/s10529-021-03080-7
Abstrakt: Objective: The extraction of the hemicellulose fraction of sugarcane bagasse (SCB) by acid hydrolysis was evaluated in an autoclave and a Parr reactor aiming the application of the hydrolysate as a carbon source for lipid production by Lipomyces starkeyi.
Results: The hydrolysis that resulted in the highest sugar concentration was obtained by treatment in the Parr reactor (HH R ) at 1.5% (m/v) H 2 SO 4 and 120 °C for 20 min, reaching a hemicellulose conversion of approximately 82%. The adaptation of the yeast to the hydrolysate provided good fermentability and no lag phase. The fermentation of hemicellulose-derived sugars (HH R ) by L. starkeyi resulted in a 27.8% (w/w) lipid content and Y P/S of 0.16 g/l.h. Increasing the inoculum size increased the lipid content by approximately 61%, reaching 44.8% (w/w).
Conclusion: The hemicellulose hydrolysate from SCB is a potential substrate for L. starkeyi to produce lipids for biodiesel synthesis based on the biorefinery concept.
Databáze: MEDLINE
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