Proteoglycans regulate protein tyrosine phosphatase receptor σ organization on hematopoietic stem/progenitor cells.

Autor: Termini CM; Division of Hematology/Oncology, Department of Medicine, University of California at Los Angeles, Los Angeles, CA; Department of Orthopaedic Surgery, University of California at Los Angeles, Los Angeles, CA; Department of Medicine, Cedars Sinai Medical Center, Los Angeles, CA., Pang A; Division of Hematology/Oncology, Department of Medicine, University of California at Los Angeles, Los Angeles, CA; Department of Medicine, Cedars Sinai Medical Center, Los Angeles, CA., Batton DM; Division of Hematology/Oncology, Department of Medicine, University of California at Los Angeles, Los Angeles, CA., Chute JP; Division of Hematology/Oncology, Department of Medicine, University of California at Los Angeles, Los Angeles, CA; Department of Orthopaedic Surgery, University of California at Los Angeles, Los Angeles, CA; Department of Medicine, Cedars Sinai Medical Center, Los Angeles, CA; Broad Stem Cell Research Center, University of California at Los Angeles, Los Angeles, CA; Jonsson Comprehensive Cancer Center, University of California at Los Angeles, Los Angeles, CA. Electronic address: john.chute@cshs.org.
Jazyk: angličtina
Zdroj: Experimental hematology [Exp Hematol] 2021 Apr; Vol. 96, pp. 44-51. Date of Electronic Publication: 2021 Jan 27.
DOI: 10.1016/j.exphem.2021.01.005
Abstrakt: Protein tyrosine phosphatase receptor σ (PTPσ) is highly expressed by murine and human hematopoietic stem cells (HSCs) and negatively regulates HSC self-renewal and regeneration. Previous studies of the nervous system suggest that heparan sulfate proteoglycans can inactivate PTPσ by clustering PTPσ receptors on neurons, but this finding has yet to be visually verified with adequate resolution. Here, we sought to visualize and quantify how heparan sulfate proteoglycans regulate the organization and activation of PTPσ in hematopoietic stem/progenitor cells (HSPCs). Our study illustrates that syndecan-2 promotes PTPσ clustering, which sustains phospho-tyrosine and phospho-ezrin levels in association with augmentation of hematopoietic colony formation. Strategies that promote clustering of PTPσ on HSPCs may serve to powerfully augment hematopoietic function.
Competing Interests: Conflict of interest disclosure The authors declare no competing financial interests.
(Copyright © 2021. Published by Elsevier Inc.)
Databáze: MEDLINE
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