α-Tocopherol Acetate Attenuates Mitochondrial Oxygen Consumption and Maintains Primitive Cells within Mesenchymal Stromal Cell Population.

Autor: Loncaric D; Etablissement Français du Sang Nouvelle Aquitaine, Place Amélie Raba Léon, CS 21010 33075, Bordeaux Cedex, France.; INSERM Bordeaux UMR 1035, Bordeaux, France.; Université de Bordeaux, Bordeaux, France., Rodriguez L; Etablissement Français du Sang Nouvelle Aquitaine, Place Amélie Raba Léon, CS 21010 33075, Bordeaux Cedex, France.; INSERM Bordeaux UMR 1035, Bordeaux, France.; Université de Bordeaux, Bordeaux, France., Debeissat C; Etablissement Français du Sang Nouvelle Aquitaine, Place Amélie Raba Léon, CS 21010 33075, Bordeaux Cedex, France.; INSERM Bordeaux UMR 1035, Bordeaux, France.; Université de Bordeaux, Bordeaux, France., Touya N; Etablissement Français du Sang Nouvelle Aquitaine, Place Amélie Raba Léon, CS 21010 33075, Bordeaux Cedex, France.; INSERM Bordeaux UMR 1035, Bordeaux, France.; Université de Bordeaux, Bordeaux, France., Labat V; Etablissement Français du Sang Nouvelle Aquitaine, Place Amélie Raba Léon, CS 21010 33075, Bordeaux Cedex, France.; INSERM Bordeaux UMR 1035, Bordeaux, France.; Université de Bordeaux, Bordeaux, France., Villacreces A; INSERM Bordeaux UMR 1035, Bordeaux, France.; Université de Bordeaux, Bordeaux, France., Bouzier-Sore AK; University Bordeaux, CNRS, CRMSB, UMR 5536, F-33000, Bordeaux, France., Pasquet JM; INSERM Bordeaux UMR 1035, Bordeaux, France.; Université de Bordeaux, Bordeaux, France., Brunet de la Grange P; Etablissement Français du Sang Nouvelle Aquitaine, Place Amélie Raba Léon, CS 21010 33075, Bordeaux Cedex, France.; INSERM Bordeaux UMR 1035, Bordeaux, France.; Université de Bordeaux, Bordeaux, France., Vlaski-Lafarge M; Etablissement Français du Sang Nouvelle Aquitaine, Place Amélie Raba Léon, CS 21010 33075, Bordeaux Cedex, France.; INSERM Bordeaux UMR 1035, Bordeaux, France.; Université de Bordeaux, Bordeaux, France., Pavlovic S; Institute for Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia., Ivanovic Z; Etablissement Français du Sang Nouvelle Aquitaine, Place Amélie Raba Léon, CS 21010 33075, Bordeaux Cedex, France. zoran.ivanovic@efs.sante.fr.; INSERM Bordeaux UMR 1035, Bordeaux, France. zoran.ivanovic@efs.sante.fr.
Jazyk: angličtina
Zdroj: Stem cell reviews and reports [Stem Cell Rev Rep] 2021 Aug; Vol. 17 (4), pp. 1390-1405. Date of Electronic Publication: 2021 Jan 28.
DOI: 10.1007/s12015-020-10111-9
Abstrakt: We present here the data showing, in standard cultures exposed to atmospheric O 2 concentration, that alpha-tocopherol acetate (α-TOA) has a positive impact on primitive cells inside mesenchymal stromal cell (MstroC) population, by maintaining their proliferative capacity. α-TOA decreases the O 2 consumption rate of MStroC probably by impacting respiratory chain complex II activity. This action, however, is not associated with a compensatory increase in glycolysis activity, in spite of the fact that the degradation of HIF-1α was decreased in presence of α-TOA. This is in line with a moderate enhancement of mtROS upon α-TOA treatment. However, the absence of glycolysis stimulation implies the inactivity of HIF-1α which might - if it were active - be related to the maintenance of stemness. It should be stressed that α-TOA might act directly on the gene expression as well as the mtROS themselves, which remains to be elucidated. Alpha-tocopherol acetate (α-TOA), a synthetic vitamin E ester, attenuates electron flow through electron transport chain (ETC) which is probably associated with a moderate increase in mtROS in Mesenchymal Stromal Cells. α-TOA action results in enhancement of the proliferative capacity and maintenance of the differentiation potential of the mesenchymal stem and progenitor cells.
(© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC part of Springer Nature.)
Databáze: MEDLINE
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